Plant microRNAs : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Stefan de Folter.
• Editors: Folter, Stefan de
• Contents: 1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants Alma Armenta-Medina and C. Stewart Gillmor 2. The Plant MicroRNA Pathway: The Production and Action Stages Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens 3. Plant miRNA Conservation and Evolution Lydia Gramzow and Günter Theißen 4. Bioinformatic Analysis of Small RNA Sequencing Libraries Ricardo A. Chávez Montes, Fabiola Jaimes Miranda, and Stefan de Folter 5. An Overview of miRNA and miRNA Target Analysis Tools Anna Lukasik and Piotr Zielenkiewicz 6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma 7. Prediction of Plant miRNA Targets Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey 8. Isolation and Detection Methods of Plant miRNAs Pedro Fernando Vera Hernández, Stefan de Folter, and Flor de Fátima Rosas Cárdenas 9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants Carlos De la Rosa and José Luis Reyes 10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska 11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization Marcelino Martínez Núñez, Stefan de Folter, and Flor de Fátima Rosas Cárdenas 12. In Situ Localization of Small RNAs in Plants Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans 13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs Andrea Tovar-Aguilar, Karla A. Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I. González-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and Noé Durán-Figueroa 14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy Andrea Gómez-Felipe and Stefan de Folter 15. Degradome Sequencing in Plants Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu 16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System Damien Formey, José Ángel Martín-Rodríguez, and Georgina Hernández 17. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis Gigi Wong and Anthony A. Millar 18. Mimicry Technology: A Versatile Tool for Small RNA Suppression Luis Manuel Villar-Martin and Ignacio Rubio-Somoza 19. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants Alberto Carbonell 20. Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches Belen Moro, Arantxa M.L Rojas, and Javier F. Palatnik 21. Analysis of Methylation Status of Plant MicroRNAs Susu Chen and Guodong Ren 22. Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 Laura Arribas-Hernández, Maria Louisa Vigh, and Peter Brodersen 23. The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs Shengben Li 24. Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae Gloria León-Martínez, Edgar Demesa-Arévalo, and Jean-Philippe Vielle-Calzada 25. Detection of Histone Modifications Associated with miRNAs Sara Hernández-Castellano and Clelia De-la-Peña.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2019
• Extent: 1 online resource (xii, 363 pages), illustrations (some color)
• Subjects: 581.3/5; Plant genetics -- Laboratory manuals; MicroRNA -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493990429
• Related ISBNs: 149399042X; 9781493990412
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed February 5, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.