Activity-based protein profiling. (2019)

• Record Type: Book
• Further Information: Note: Editors, Benjamin F. Cravatt, Ku-Lung Hsu and Eranthie Weerapana.
• Editors: Cravatt, Benjamin F; Hsu, Ku-Lung; Weerapana, Eranthie
• Contents: Intro; Preface; Contents; 128 Activity-Based Protein Profiling-Enabling Multimodal Functional Studies of Microbial Communities; Abstract; 1 Introduction; 2 Probe Design; 2.1 Activity- and Affinity-Based Probes for Multimodal Characterization of Microbiome Function; 2.2 Click Chemistry and Sample Analysis; 3 Challenges of Microbiome Proteomics and How ABPP Can Facilitate; 4 Biological Applications for ABPP; 4.1 Host-Associated Microbial Communities; 4.1.1 Xenobiotic Metabolism; 4.1.2 Bile Acid Metabolism and Signaling; 4.2 Environmental Microbial Communities 4.2.1 Metabolic Activity and Translation4.2.2 Vitamin Metabolism; 4.2.3 Community Signaling; 5 Current Challenges and Outlook; References; 135 Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles; Abstract; 1 Introduction; 1.1 Activity-Based Probes: What They Are and How They Work; 2 Probe Design Strategies; 2.1 Reactive Warhead and Recognition Elements; 2.2 Linker; 2.3 Reporter; 2.3.1 Quenched Activity-Based Probes; 2.3.2 Bioorthogonal Ligation Reactions; 3 Detection Methods; 3.1 Gel-Based Detection; 3.2 Live-Cell Imaging 3.3 Mass Spectrometry4 ABPP in Bacteria; 4.1 Serine-Reactive Probes; 4.2 Cysteine-Reactive Probes; 4.3 Antibiotic-Derived Probes; 4.4 Carbohydrate-Based Probes; 4.5 Probes Inspired by Natural Products; 5 Application of ABPP in Natural Product Target Discovery; 6 Conclusion; References; 125 Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria; Abstract; 1 Introduction; 2 Activity-Based Protein Profiling in Mycobacterium tuberculosis; 2.1 Profiling of ATP-Binding Proteins; 2.2 Profiling of Serine Hydrolase Enzymes 2 Applications of ABPP to In Vitro Models of Microbial Infection2.1 Detecting Host Enzymes Active in Infected Cells; 2.2 Profiling Pathogen Adaptation to the Host Environment; 3 Applications of ABPP to Infected Plants; 3.1 Discovery of Pathogen-Secreted Protease Inhibitors; 3.2 Global Profiling of Active Enzymes; 3.3 Mechanistic Studies of Proteasome Activity and Inhibition; 4 Applications of ABPP to Infected Animals; 5 Applications of ABPP to Inhibitor Discovery and Characterization; 5.1 Identifying the Targets of Bioactive Inhibitors; 5.2 High-Throughput Screens for Selective Inhibitors
• Publisher Details: Cham, Switzerland : Springer
• Publication Date: 2019
• Extent: 1 online resource
• Subjects: 572/.6; Proteomics; Proteins; SCIENCE / Life Sciences / Biochemistry; Electronic books; Electronic books
• Languages: English
• ISBNs: 9783030111434; 3030111431
• Related ISBNs: 9783030111427; 3030111423
• Notes: Note: Includes bibliographical references.; Note: Online resource; title from PDF title page (EBSCO, viewed January 31, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.