Calcium Signalling : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Anna Raffaello, Denis Vecellio Reane.
• Editors: Raffaello, Anna; Vecellio Reane, Denis
• Contents: Preface… Table of Contents… Contributing Authors…1. High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels Simona Feno, Giulia di Marco, Agnese di Mario, Halenya Monticelli, and Denis Vecellio Reane 2. Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling Luisa Galla, Paola Pizzo, and Elisa Greotti 3. Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators Mattia Vicario and Tito Calì 4. Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes Sofia Zanin, Elisa Lidron, and Giorgia Pallafacchina 5. MCU Regulation in Lipid Bilayer and Electrophysiological Recording Vanessa Checchetto and Ildikò Szabò 6. Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer Vanessa Checchetto and Ildikò Szabò 7. Patch Clamp Analysis of the Mitochondrial Calcium Uniporter Vivek Garg and Yuriy Y. Kirichok 8. In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana Neli Romano Armada, Fabrizio Gandolfo Doccula, Alessia Candeo, Gianluca Valentini, Alex Costa, and Andrea Bassi 9. Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes Gaia Gherardi and Cristina Mammucari 10. Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes Antonio Campo and Marco Mongillo 11. In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle Rüdiger Rudolf, Sofie Trajanovska, David Grant Allen, and Tullio Pozzan 12. TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis Simone Di Paola and Diego L. Medina 13. Ca2+-Dependent Regulation of TFEB and Lysosomal Function Simone Di Paola and Diego L. Medina 14. Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum Lucas Borges-Pereira and Célia R. S. Garcia 15. Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2 Martin Johnson 16. Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes Gabriele Losi, Anna Maria Lia, Marta Gomez-Gonzalo, Micaela Zonta, and Giorgio Carmignoto 17. Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes Christine Gibhardt, Adina Vultur, and Ivan Bogeski 18. Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer Jennifer Wettmarshausen and Fabiana Perocchi 19. Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells Michela Menegollo, Isabella Tessari, Luigi Bubacco, and Gyorgy Szabadkai 20. Purification of Functional F-ATP Synthase from Blue Native PAGE Chiara Galber, Giulia Valente, Sophia von Stockum, and Valentina Giorgio.
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (246 pages)
• Subjects: Life sciences; Biochemistry; Science -- Life Sciences -- Biochemistry; Biochemistry
• Languages: English
• ISBNs: 9781493990184
• Related ISBNs: 9781493990177
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.