Autophagy : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Nicholas Ktistakis and Oliver Florey.
• Editors: Ktistakis, Nicholas T; Florey, Oliver
• Contents: Part I: Autophagry In Vitro 1. Recombinant Expression, Purification and Assembly of p62 Filaments Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse 2. Structural Studies of Autophagy-related Proteins Melanie Schwarten, Oliver H. Weiergräber, Dušan Petrović, Birgit Strodel, and Dieter Willbold 3. Structural Studies of Mammalian Autophagy Lipidation Complex Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo 4. Structural Studies of Selective Autophagy in Yeast Akinori Yamasaki, Yasunori Watanabe and Nobuo N. Noda 5. Biophysical Studies of LC3 Family Proteins Javier H. Hervás, Zuriñe Antón, and Alicia Alonso 6. Reconstituting Autophagy Initiation from Purified Components Peter Mayrhofer and Thomas Wollert 7. Cell-free Reconstitution of Autophagic Membrane Formation Min Zhang and Liang Ge 8. Use of Peptide Arrays for Identification and Characterization of LIR Motifs Mads Skytte Rasmussen, Åsa Birna Birgisdottir and Terje Johansen 9. Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System Yang Chen, Qian Peter Su, and Li Yu 10. Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature Jaime Agudo-Canalejo and Roland L. Knorr 11. Studies of Receptor-Atg8 Interactions during Selective Autophagy Christine Abert and Sascha Martens Part II: Imaging Autophagy in Tissue Culture 12. Correlative Light and Electron Miscopy of Autophagosomes Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen 13. Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells Ritsuko Arai and Satoshi Waguri 14. 3-color Simultaneous Live Imaging of Autophagy-related Structures Maho Hamasaki, Hiroyuki Ueda, Ouin Kunitaki, and Tamotsu Yoshimori 15. Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy Eleftherios Karanasios 16. Methods for Imaging Autophagosome Dynamics in Primary Neurons Audrey Dong, Vineet Vinay Kulkarni, and Sandra Maday 17. Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research Stathakos, P., Jimenez-Moreno, N., Crompton, L., Nistor, P, Caldwell, M.A., and Lane, J.D 18. Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo Céline Largeau and Renaud Legouis 19. Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells Elise Jacquin, Katherine Fletcher, and Oliver Florey Part III: Assays to Measure (and Screen For) Autophagy and Related Processes 20. Measurement of Bulk Autophagy by a Cargo Sequestration Assay Nikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen 21. Methods to Detect Loss of Lysosomal Membrane Integrity Sonja Aits 22. ­­Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress Maria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont 23. Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches Stéphanie Kaeser-Pebernard, Britta Diedrich, and Jörn Dengjel 24. Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze 25. Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening Martina Wirth1 and Sharon A. Tooze 26. In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells Elena Seranova, Carl Ward, Miruna Chipara, Ratul Banerjee, Tatiana R. Rosenstock, and Sovan Sarkar 27. In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells Elena Seranova, Carl Ward, Miruna Chipara, Ratul Banerjee, Tatiana R. Rosenstock, and Sovan Sarkar 28. Methods for the Study of Entotic Cell Death Jens C. Hamann, Sung Eun Kim, and Michael Overholtzer 29. MHC Class I Internalization via Autophagy Proteins Monica Loi, Laure-Anne Ligeon and Christian Münz Part IV: Measuring and Imaging Autophagy In Vitro 30. Analysis of Autophagy for Liver Pathogenesis Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin 31. Autophagy in 3D In Vitro and Ex Vivo Cancer Models Carlo Follo, Dario Barbone, William G. Richard2, Raphael Bueno, and V. Courtney Broaddus 32. Autophagy in Platelets Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, and Qing Jun Wang 33. Methods to Image Macroautophagy in the Brain In Vivo Xigui Chen, Kanoh Kondo, and Hitoshi Okazawa 34. Measuring Nonselective and Selective Autophagy in the Liver Takashi Ueno and Masaaki Komatsu 35. Measuring Autophagy in Pancreatitis Alejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro 36. Characterization of the 'autophagic flux' in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy Kalamida D., Giatromanolaki A., and Koukourakis M.I 37. Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing Sivan Henis-Korenblita and Alicia Meléndez Part V: Mitophagy and Other Selective Autophagy Pathways 38. Investigating Non-selective Autophagy in Drosophila Szabolcs Takáts, Sarolta Tóth, Győző Szenci, and Gábor Juhász 39. Imaging the Dynamics of Mitophagy in Live Cells Andrew S. Moore and Erika L.F. Holzbaur 40. Triggering Mitophagy with Photosensitizers Cheng-Wei Hsieh and Wei Yuan Yang 41. Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC Thomas G. McWilliams and Ian G. Ganley 42. Assays to Monitor Mitophagy in Drosophila Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P. Nezis 43. Mitophagy Dynamics in Caenorhabditis elegans Konstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis 44. Methods for Studying Mitophagy in Yeast Panagiota Kolitsida and Hagai Abeliovich 45. Measuring Anti-bacterial Autophagy Keith B Boyle and Felix Randow 46. Quantitative Phosphoproteomics of Selective Autophagy Receptors Thomas Juretschke, Petra Beli, and Ivan Dikic 47. Analysis of Chaperone-mediated Autophagy Y.R. Juste and A.M. Cuervo 48. Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations Jana Petri and Roland L. Knorr.
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (741 pages)
• Subjects: 571.936; Cell death; Apoptosis; Homeostasis
• Languages: English
• ISBNs: 9781493988730
• Related ISBNs: 9781493988723
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.