T-cell motility : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Navin Kumar Verma.
• Editors: Verma, Navin Kumar
• Contents: Preface… Table of Contents… Contributing Authors… 1. An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility Navin Kumar Verma and Dermot Kelleher 2. Isolation of Human Peripheral Blood T Lymphocytes Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma 3. A Laboratory Model to Study T-Cell Motility Navin Kumar Verma, Sjyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher 4. Quantification of T-Cell Migratory Phenotypes using High Content Analysis Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma 5. Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time Seow Theng Ong and Navin Kumar Verma 6. Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma 7. Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis Praseetha Prasannan and Navin Kumar Verma 8. Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes Atish Kizhakeyil and Navin Kumar Verma 9. GapmeR-Mediated Gene Silencing in Motile T-Cells Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Chalasani, Atish Kizhakeyil, and Navin Kumar Verma 10. Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann 11. Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma 12. Immunometabolomic Phenotyping of Motile T-Cells Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma 13. Profiling Activity of Cellular Kinases in Migrating T-Cells Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and Wim Vanden Berghe 14. Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes Malin Samuelsson and Lena M. Svensson 15. A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy Mobashar Hussain Urf Turabe Fazil, Pankal Kumar, and Navin Kumar Verma 16. Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma 17. Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers 18. Computational Analysis of Protein-Protein Interactions in Motile T-Cells Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khursheed Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma.
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (158 pages)
• Subjects: 616.0797; T cells
• Languages: English
• ISBNs: 9781493990368
• Related ISBNs: 9781493990351
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.