Soft Probes for Bio-Electrochemical Imaging. (2018)

• Record Type: Book
• Other Names: Lin, Tzu-En
• Contents: Intro; Supervisor's Foreword; Abstract; Preface; Parts of this thesis have been published in the following journal articles:; Contents; Abbreviations; Symbols; 1 Introduction; 1.1 Theory and Principles of Scanning Electrochemical Microscopy (SECM); 1.1.1 The Origin of SECM; 1.1.2 Instrumentation of SECM; 1.1.3 Main Operation Modes of SECM; 1.1.4 SECM Applied to Biological Samples; 1.1.5 Limitations of SECM for Real Samples and Approaches to Overcome Them; 1.1.6 Soft-Probe-SECM; 1.2 Melanoma; 1.2.1 Melanoma Classification; 1.2.2 Tyrosinase and Its Enzymatic Reactions 1.2.3 Melanoma Diagnosis and Detection Methods1.3 Bio-application of Graphene-Based Nanomaterials; 1.4 Thesis Outline; References; 2 Experimental Part; 2.1 Preparation of GONRs, PEG-GONRs and PEG-MNP-GONRs; 2.2 Sample Preparations; 2.2.1 Immobilization of Biological and Nanomaterials on Polyvinylidene Difluoride (PVDF) Membranes; 2.2.2 Preparation of Tissue, Organ and Fruit Samples; 2.2.3 Fruit Sample Preparation; 2.3 Three-Dimensional, Inkjet Printed Test Samples; 2.4 Conventional and Soft-Probe-SECM; 2.4.1 Preparation of Conventional Pt Electrode 2.4.2 Soft Stylus Probes Manufacturing for Soft-Probe-SECM2.4.3 SECM Instrumentation; 2.5 SECM Measurements and General Measurements Procedures; 2.5.1 Indirect SECM Detection of Adsorbed Proteins on PVDF Membrane by Oxygen Reduction Method; 2.5.2 SECM Detection of Tyrosinase Activity Immobilized on PVDF Membrane; 2.5.3 Immunoassay Strategy; 2.5.4 SECM Images of Tyrosinase in Thin Tissue Sections; 2.5.5 Immunohistochemistry; 2.6 Single-Photon Emission Computed Tomography (SPECT)/Computed Tomography (CT); Annex; References 3 Multiple SECM Mapping of Tyrosinase in Micro-contact Printed Fruit Samples on Polyvinylidene Fluoride Membrane3.1 Introduction; 3.2 Results and Discussion; 3.2.1 Indirect SECM Detection of Adsorbed Proteins on PVDF Membrane by Oxygen Reduction Method; 3.2.2 Detection of Tyrosinase Enzymatic Activity by SECM; 3.2.3 Mass Spectrometry Analysis of the Enzymatic Reaction; 3.2.4 SECM Immunoassay of Tyrosinase Immobilized on PVDF Membrane; 3.2.5 SECM of µCP Banana on PVDF Membrane; 3.3 Conclusions; References 4 Monitoring Tyrosinase Expression in Non-metastatic and Metastatic Melanoma Tissues by SECM4.1 Introduction; 4.2 Additional Experimental Details; 4.3 Results and Discussions; 4.4 Conclusion; References; 5 From Tissues to Organs: Soft Electrochemical Probes for Scanning Human and Animal Tissues; 5.1 Introduction; 5.2 Additional Experimental Details of Soft Probe Fabrication; 5.3 Results and Discussions; 5.3.1 Spider Probe Characterization; 5.3.2 Soft Single Probe on Thick Melanoma Tissues; 5.3.3 Thick Melanoma Tissue Scanning by Spider Probe; 5.3.4 Scanning an Entire Mouse Heart Section
• Publisher Details: Cham : Springer
• Publication Date: 2018
• Extent: 1 online resource (136 pages)
• Subjects: 502.8/2; Scanning electrochemical microscopy; Biochemical markers; Biochemical markers; Scanning electrochemical microscopy; Electronic books; Electronic books
• Languages: English
• ISBNs: 9783030057589; 3030057585
• Related ISBNs: 9783030057572; 3030057577
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.