X-ray free electron lasers : a revolution in structural biology /: a revolution in structural biology. ([2018])

• Record Type: Book
• Further Information: Note: Sébastien Boutet, Petra Fromme, Mark S. Hunter, editors.
• Editors: Boutet, Sébastien; Fromme, Petra; Hunter, Mark S
• Contents: Intro; Preface; Contents; Contributors; 1 X-Ray Free Electron Lasers and Their Applications; 1.1 X-Rays and Their Applications; 1.1.1 X-Ray Methods; 1.1.1.1 Scattering; 1.1.1.2 Spectroscopy; 1.1.2 The Evolution of X-Ray Sources; 1.2 X-Ray FELs as User Facilities; 1.2.1 The Physics of Free Electron Lasers; 1.3 The Scientific Applications of X-Ray FELs; 1.3.1 Using the Time Resolution; 1.3.2 Using the High Peak Intensity; 1.3.2.1 Diffraction-Before-Destruction; 1.3.2.2 Using FELs to Create New States of Matter; 1.3.2.3 Nonlinear X-Ray Physics; 1.3.3 Using the Coherence; 1.4 Summary; References 2 Serial Femtosecond Crystallography (SFX): An Overview2.1 Background; 2.2 The Need for SFX; 2.2.1 Sample Preparation and Characterization for SFX; 2.2.2 Sample Introduction for SFX; 2.2.3 Data Analysis for SFX; 2.3 Early Experiments of Serial Femtosecond Crystallography (SFX); 2.3.1 The "Birth" of SFX; 2.3.2 SFX at High Resolution; 2.3.3 SFX of G-Protein Coupled Receptors; 2.3.4 Phasing of SFX Data; 2.3.5 Time-Resolved SFX; 2.3.6 Imperfect Crystals and the Push Toward Crystal-Free Imaging; 2.3.7 Towards Single Particle X-ray Diffraction 2.3.8 Structure Determination Based on fs Small and Wide Angle Solution X-ray Scattering2.4 Pushing SFX Forward; 2.4.1 Serial Operations at Beamlines for SFX at Current X-ray FELs; 2.4.2 New Experimental Endstations for X-ray FEL Experiments; 2.4.3 Higher Repetition Rate X-ray FELs; 2.5 Outlook; References; 3 Small Is Beautiful: Growth and Detection of Nanocrystals; 3.1 Introduction; 3.2 Nanocrystallogenesis; 3.2.1 Thermodynamics of Solubility and Nucleation; 3.2.2 Methods; 3.2.2.1 Adaptation of Existing Conditions; 3.2.2.2 Free Interface Diffusion; 3.2.2.3 Batch Nanocrystallization 3.2.2.4 Other Methods and Sample Delivery Considerations3.2.3 Stability and Storage; 3.3 Considerations and Characterization for SFX Optimization; 3.3.1 Characteristics: What Is Optimal?; 3.3.1.1 Data Analysis Considerations; 3.3.1.2 Practicality: Sample and Hardware; 3.3.1.3 Control of Homogeneity Through Post Growth Methods; 3.3.2 Characterization; 3.3.2.1 Optical Detection: Visualizing Your Crystals; 3.3.2.2 Light Scattering Techniques for Size Determination; 3.3.2.3 Transmission Electron Microscopy; 3.3.2.4 Powder Diffraction; 3.4 Recap; References 4 The Lipid Cubic Phase as a Medium for the Growth of Membrane Protein Microcrystals4.1 Introduction; 4.2 Procedure for the Preparation and Characterization of Microcrystals for LCP-SFX; 4.2.1 Membrane Protein Reconstitution in LCP (Modified from Ref. [28]); 4.2.2 Sample Consolidation and Titration With 7.9 MAG; 4.2.3 Microcrystal Characterization; 4.2.4 Adjusting Crystal Density; 4.2.5 Loading the Sample in an LCP Injector for LCP-SFX Data Collection; 4.3 Results; 4.4 Conclusion; References; 5 Sample Delivery Techniques for Serial Crystallography; 5.1 Overview
• Publisher Details: Cham, Switzerland : Springer
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 621.36/6; Free electron lasers; Electronic books
• Languages: English
• ISBNs: 9783030005511; 3030005518
• Related ISBNs: 9783030005504
• Notes: Note: Description based on online resource; title from digital title page (viewed on January 23, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.