Microscopy of the Heart. (2018)

• Record Type: Book
• Further Information: Note: Lars Kaestner, Peter Lipp, editors.
• Other Names: Kaestner, Lars; Lipp, Peter
• Contents: Intro; Preface; Contents; Studying Structure and Function of the Heart Cells Using Scanning Ion Conductance Microscopy; Introduction; The Technique of SICM; History; Setup; Principle; Imaging of the Heart Cell Topography by SICM; Cardiomyocytes; Endothelial Cells; Combining Cardiomyocyte Structure with Function; Super-Resolution Scanning Patch-Clamp; SICM-FRET; Scanning Laser Confocal Microscopy; SICM as a Mechanical Probe; Cell Volume Measurements by SICM; Summary; References; Optical Sectioning Microscopy at `Temporal Super-Resolution;́ General Introduction; Confocal Microscopy Single Beam OptionsMulti Beam Options; Analysis Algorithms; Examples from Cardiac Ca2+-Handling; Non-linear Microscopy; Multi Photon Microscopy; SHG Imaging; Light-Sheet Imaging; Conclusions; References; Quantitative Super-Resolution Microscopy of Cardiomyocytes; Background; Issues with Conventional Optical Imaging for Cardiac Studies; Optical Super-Resolution Imaging; Optical Super-Resolution Techniques Roundup; Applications of SMLM to Cardiac Preparations; Novel Insights into the Distribution of RyRs in Myocytes Use of Multi-Colour and Quantitative dSTORM for Resolving Further Junctional FeaturesJunctophilin-2; T-system; Methodological Considerations: Adaptating Localisation Microscopy for the Study of Cardiac Muscle; SMLM Imaging Principles: dSTORM; Fluorophores and Sample Preparation; Anatomy of an SMLM Apparatus; Image Analysis for Obtaining Super-Resolution Images; Considerations in Achieving Super-Resolution Imaging; Considerations in Quantifying Structures and Labelling Densities; Outlook; Conclusion; References; Caged Compounds: Applications in Cardiac Muscle Research; Introduction Caged Compounds: General PropertiesApplications of Caged Compounds in Cardiac Muscle Research; Ca2+ Signaling; Caged Ca2+ Compounds; Example Studies with Caged Ca2+ Compounds; L-type Ca2+ Current; Na+-Ca2+ Exchange Current; Activation of CICR; Termination of CICR; Regulation and Pathology of CICR; Muscle Contraction and Related Compounds; ATP Hydrolysis, Activation of Tension and Rigor Force Decay; ATP-Sensitive K+ Channels; Cardiac Regulatory Mechanisms and Related Compounds; Caged cAMP; Caged InsP3; Various Caged Compounds; General Considerations for the Use of Caged Compounds Light Sources and IlluminationFlash Lamps; Pulsed Lasers; Diode Lasers and Light Emitting Diodes (LED); Localized and Two-Photon Excitation; Loading of the Cells with Caged Compounds; Patch Pipette; Cell Permeant Caged Compounds; Limitations of the Technique and the Compounds; Stability and Shelf Life; Formation of Phototoxic By-Products; Absorbance and Quantum Yield; New Developments: Outlook; Enhanced Caging Groups and Probes Optimized for Two-Photon Photolysis; New Spectral Windows for Multicolor Excitation Photolysis; Optogenetics; References
• Publisher Details: Cham, Switzerland : Springer Nature Springer
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 612.1; Heart -- Microscopy; Scanning electron microscopy; MEDICAL / Physiology; SCIENCE / Life Sciences / Human Anatomy & Physiology; Heart -- Microscopy; Scanning electron microscopy; Electronic books
• Languages: English
• ISBNs: 9783319953045; 3319953044
• Related ISBNs: 9783319953021
• Notes: Note: Online resource; title from PDF file page (EBSCO, viewed December 11, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.