BCL-2 Family Proteins : Methods and Protocols /: Methods and Protocols. (2018)

• Record Type: Book
• Further Information: Note: Evripidis Gavathiotis.
• Editors: Gavathiotis, Evripidis
• Contents: Preface…Table of Contents…Contributing Authors… 1. Overview of BCL-2 Family Proteins and Therapeutic Potentials Jason D. Huska, Heather M. Lamb, and J. Marie Hardwick 2. Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins Abdel Aouacheria, Vincent Navratil, and Christophe Combet 3. Application of Mito-Priming to Generate BCL-2 Addicted Cells Jonathan Lopez and Stephen W.G. Tait 4. BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies Cameron Fraser, Jeremy Ryan, and Kristopher Sarosiek 5. Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP) Linsey M. Ludwig, Katrina L. Maxcy, and James L. LaBelle 6. Investigating BCL-2 Family Protein Interactions in Yeast Stéphen Manon 7. Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family Denis E. Reyna and Evripidis Gavathiotis 8. Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP Mark P.A. Luna-Vargas, Jarvier N. Mohammed, and Jerry E. Chipuk 9. Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family Jialing Lin, Arthur E. Johnson, and Zhi Zhang 10. Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol Joachim Lauterwasser, Franziska Fimm-Todt, and Frank Edlich 11. Quantification of BCL-2 Family Members by Flow Cytometry Morey L. Smith and Stephen K. Tahir 12. Methods to Probe Calcium Regulation by BCL-2 Family Members Marcos A. Carpio and Samuel G. Katz 13. Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK Geetika Singh and Tudor Moldoveanu 14. Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage Sweta Iyer, Rachel T. Uren, and Ruth M. Kluck 15. BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy Thomas P. Garner and Evripidis Gavathiotis 16. Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs Yong Yao and Francesca M. Marassi 17. Cryo-Electron Microscopy to Study Bax Pores and MOMP Tomomi Kuwana 18. CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores Tirtha Mandal, Eric J. Hustedt, Likai Song, and Kyoung Joon Oh 19. Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET Elizabeth J. Osterlund, Nehad Hirmiz, Christian Tardiff, and David W. Andrews 20. Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy Fabronia Murad and Ana J. Garcia-Saez 21. Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX) Zhou Dai and Jonathan R. Lai.
• Publisher Details: New York : Humana Press
• Publication Date: 2018
• Copyright Date: 2019
• Extent: 1 online resource (360 pages)
• Subjects: Life sciences; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins
• Languages: English
• ISBNs: 9781493988617
• Related ISBNs: 9781493988600
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.