Tumor microenvironment : study protocols /: study protocols. (2016)

• Record Type: Book
• Further Information: Note: Constantinos Koumenis, Lisa M. Coussens, Amato Giaccia, Ester Hammond.
• Editors: Koumenis, Constantinos; Coussens, Lisa M; Giaccia, Amato J; Hammond, Ester
• Contents: 1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells.- 2.Measuring DNA replication rates in hypoxic conditions.- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions.- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis.- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells.- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy.- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells.- 8.Measuring Autophagy in the Context of Cancer.- 9.Methods for studying autophagy within the tumor microenvironment.- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia.- 11.Isolation of Glioma Initiating Cells for Biological Study.- 12.Isolation of mouse and human tumor associated macrophages.- 13.Mouse models of brain metastasis for unravelling tumour progression.- 14.Quantification of lung metastases from in vivo mouse models.- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA).- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.
• Publisher Details: Switzerland : Springer
• Publication Date: 2016
• Extent: 1 online resource (viii, 297 pages), illustrations (some color)
• Subjects: 616.9/94; Medicine; Cancer -- Molecular aspects; Tumors -- Molecular aspects; HEALTH & FITNESS / Diseases / General; MEDICAL / Clinical Medicine; MEDICAL / Diseases; MEDICAL / Evidence-Based Medicine; MEDICAL / Internal Medicine; Cancer -- Molecular aspects; Science -- Life Sciences -- Biology -- Molecular Biology; Medical -- Immunology; Medical research; Immunology; Oncology; Immunology; Medical -- Oncology; Oncology; Electronic books
• Languages: English
• ISBNs: 9783319266664; 3319266667
• Related ISBNs: 9783319266640; 3319266640
• Notes: Note: Includes bibliographical references at the end of each chapter and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed June 30, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.