Electrophoretic separation of proteins : methods and protocols /: methods and protocols. ([2019])

• Record Type: Book
• Further Information: Note: Edited by Biji T. Kurien and R. Hal Scofield.
• Editors: Kurien, Biji T; Scofield, R. Hal
• Contents: Preface…Table of Contents…Contributing Authors…1. How It All Began: A Personal History of Gel-ElectrophoresisOliver Smithies2. Introduction to Protein ElectrophoresisPothur R. Srinivas3. Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic EvaluationJ.P. Dean Goldring4. Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein ElectrophoresisEwa I. Miskiewicz and Daniel J. MacPhee5. The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS ElectrophoresisThierry Rabilloud6. Cellulose-Acetate Electrophoresis of HemoglobinRamesh Kumar and Wilbert A. Derbigny7. Native Polyacrylamide gelsClaudia Arndt, Stefanie Koristka, Anja Feldmann, and Michael Bachmann8. Isoelectric Focusing on Non-Denaturing GelsBiji T. Kurien and R. Hal Scofield9. Determination of protein Molecular weights on SDS-PAGEHiroyuki Matsumoto, Hisao Haniu, and Naoka Komori10. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGEHiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, and Maoka Komori11. Cationic ElectrophoresisEngelbert Buxbaum12. Two-Dimensional Gel Electrophoresis with Immobilized pH GradientsBre'Ana Byrd and Huyen Tran13. SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated ProteinsChristian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, and Wilhelm Schänzer14. Tricine-SDS-PAGESyed R. Haider, Helen J. Reid, and Barry L. Sharp15. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel ElectrophoresisMarta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, and Jean M. H. Van den Elsen16. Immunofixation Electrophoresis for Identification of Proteins and Specific AntibodiesGyorgy Csako17. Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-AgaroseMarion L. Greaser and Chad M. Warren18. Increase in Local Protein Concentration by Field-Inversion Gel ElectrophoresisHenghang Tsai and Hon-chiu Eastwood Leung19. Two-Dimensional Difference Gel ElectrophoresisMalachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden20. Immunoelectrophoresis: A Method with Many FacesGyorgy Csako21. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular MassMonica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa22. Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide BondsRonald Saraswat and Brian McDonagh23. Identification of Proteins on Archived 2D GelsHiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori24. Two-Dimensional Gel Electrophoresis: Vertical Isoelectric FocusingYaser Dorri25. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary PolyphenolsMustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes26. Characterization of New Cyclic D, L – α – Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass SpectrometryMaría Dámaris Cortez-Díaz, Fanny d'Orlyé, and Anne Varenne27. 'Microchip Electrophoresis', with Respect to 'Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheier's Disease'Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy28. Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility ShiftKaren A. Lewis, Sarah E. Altschuler, and Deborah S. Wuttke29. Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down AssayBhupinder Singh and Swapan K. Nath30. Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA ComplexesJennifer A. Ream, L. Kevin Lewis, and Karen A. Lewis31. Applications of Immobilized Metal affinity ElectrophoresisBao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta32. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological FluidsGyorgy Csako33. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological SamplesKim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann34. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western BlottingEdward P. Trieu and Ira N. Targoff35. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and CharacterizationMing Dong, Megan Choi, Mark D. Biggin, and Jian Jin36. Cell Surface Protein Biotinylation for SDS-PAGE AnalysisGiuliano Elia37. Isolation of Proteins from Polyacrylamide GelsStefanie Koristka, Claudia Arndt, Ralf Bergmann, and Michael Bachmann38. Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex MixturesRobert G.E. Krause and J.P. Dean Goldring39. Protein Extraction from Gels: A Brief ReviewBiji T. Kurien, Rachna Aggarwal, and R. Hal Scofield40. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane ProteomeXianchun Wang and Songping Liang41. Ultra-Rapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel ElectrophoresisRohit Thomas and Biji T. Kurien42. A Brief Review of Other Notable Electrophoretic MethodsJames D. Fesmire43. Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge UnitKristi A. Koelsch44. Artifacts and Common Errors in Protein Gel ElectrophoresisBiji T. Kurien and R. Hal Scofield.
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2019
• Extent: 1 online resource (xv, 523 pages), illustrations (some color)
• Subjects: 541/.372; Life sciences; Electrophoresis -- Laboratory manuals; Proteins -- Separation -- Laboratory manuals; Electrophoresis; Proteins -- Separation; Electrochemical Techniques; Electrophoresis -- methods; Chemistry Techniques, Analytical; Electrophoresis, Agar Gel; Immunoelectrophoresis; Proteins; Laboratory Manuals; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Laboratory manuals
• Languages: English
• ISBNs: 9781493987931; 1493987933
• Related ISBNs: 9781493987924
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed November 13, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.