A clinician's guide to sperm DNA and chromatin damage. (2018)

• Record Type: Book
• Further Information: Note: Armand Zini, Ashok Agrwal, editors.
• Editors: Zini, Armand; (Physician), Agarwal, Ashok
• Contents: Intro; Foreword; Preface; Contents; Editors; Contributors; Part I: Human Sperm Chromatin Structure; Chapter 1: Sperm Chromatin: An Overview; 1.1 Sperm Chromatin: The First 50 Years; 1.2 Spermatogenesis: Terminal Differentiation and Reprogramming of the Testicular Cell Genome; 1.3 Chromatin Reorganization in Maturing Spermatids; 1.4 Protamines P1 and P2; 1.5 Protamine-DNA Interactions and Structure of the Complex; 1.6 Higher-Ordered Organization of Chromatin in Mature Sperm; 1.7 Preservation of DNA Domains and Nuclear Matrix Associations 1.8 Reactivation of Paternal Chromatin Following Sperm-­Oocyte Fusion1.9 Consequences of Disrupting Sperm Chromatin Remodeling; References; Chapter 2: Sperm Nucleoproteins (Histones and Protamines); 2.1 Introduction; 2.2 Nucleoprotamine Complex in Sperm; 2.2.1 Protamine Post-translational Modifications; 2.2.2 Protamine Alterations in Infertile Patients; 2.3 Nucleohistone Complex in Sperm; 2.3.1 Histone Variants; 2.3.2 Histone Post-translational Modifications; 2.3.3 Histone-Bound Sperm Chromatin; 2.3.4 Histone Alterations and Male Infertility; 2.4 Concluding Remarks; References Chapter 3: Sperm Nuclear Architecture3.1 Introduction; 3.2 A Model; 3.3 Sperm Nuclear Matrix; 3.4 Sperm Chromosomes; 3.5 Conclusions; References; Part II: Laboratory Evaluation of Sperm Chromatin and DNA Damage; Chapter 4: Sperm Chromatin Structure Assay (SCSA®): Evolution from Origin to Clinical Utility; 4.1 Origin, Standardization, and Verification of the SCSA Test as Marker of Male Sub-/Infertility; 4.1.1 Origin of the SCSA Test; 4.1.2 Standardization of the SCSA Test: Changes to the Finalized and Federal Registered Protocol; 4.1.2.1 Problems with the Heated Sperm Nuclei Protocol 4.1.2.2 Low pH to Denature DNA at DNA Break Sites4.1.3 Biochemistry of Acridine Orange (AO) and Sperm DNA Interactions of the SCSA Test; 4.1.4 Does the SCSA Test Measure Potential or Existing Sperm DNA Strand Breaks?; 4.1.5 Change in SCSA Terminology; 4.1.6 Clinical Report; 4.1.7 Relationship Between Sperm DNA Fragmentation Data and Classical Semen Parameters; 4.2 Biochemical Characteristics of SCSA-Defined Sperm Populations; 4.2.1 FCM Sorted SCSA Populations to Analyze Sperm Nuclear Morphology; 4.2.2 Characteristics of HDS Population: New Emphasis for the ART Lab 4.3 Validation of SCSA Clinical Thresholds4.3.1 Humans; 4.3.2 Animals; 4.3.2.1 Bulls; 4.3.2.2 Boars; 4.4 Validation of the SCSA Test for Precision and Accuracy; 4.4.1 Invaluable Use of Flow Cytometry; 4.4.2 Repeatability of SCSA Data; 4.4.2.1 Within Human Donors Over Time; 4.4.2.2 SCSA Data Using Different Flow Cytometers Internationally on Sperm from Eight Different Mammalian Species; 4.4.2.3 Comparisons Between Measurements of Aliquots of Human Patient Semen Samples on Three Continents; 4.5 SCSA Data as Related to Male Age, a Very Important Infertility Issue
• Publisher Details: Cham : Springer
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 572.8/6; Medicine; DNA damage; Chromatin; SCIENCE / Life Sciences / Biochemistry; Chromatin; DNA damage; DNA Damage; Medical -- Urology; Medical -- Endocrinology & Metabolism; Urology & urogenital medicine; Endocrinology; Reproductive Medicine; Andrology; Endocrinology; Health & Fitness -- Diseases -- Genetic; Reproductive medicine; Electronic books
• Languages: English
• ISBNs: 9783319718156; 3319718150
• Related ISBNs: 9783319718149; 3319718142
• Notes: Note: Online resource; title from PDF title page (EBSCO, viewed March 13, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.