Advanced technologies for protein complex production and characterization. (2016)

• Record Type: Book
• Further Information: Note: M. Cristina Vega, editor.
• Editors: Vega, M. Cristina
• Contents: Protein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants -- potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. .
• Publisher Details: Cham : Springer
• Publication Date: 2016
• Extent: 1 online resource (xiii, 382 pages), illustrations (some color)
• Subjects: 572/.6; Life sciences; Protein engineering; Proteins -- Biotechnology; Protein drugs; SCIENCE / Life Sciences / Biochemistry; Protein drugs; Protein engineering; Proteins -- Biotechnology; Multiprotein Complexes -- biosynthesis; Multiprotein Complexes -- chemistry; Protein Engineering; Protein Interaction Mapping -- methods; Life Sciences; Protein Science; Protein Structure; Systems Biology; Science -- Life Sciences -- Biochemistry; Science -- Life Sciences -- General; Proteins; Life sciences: general issues; Biochemistry; Biological models; Electronic books
• Languages: English
• ISBNs: 9783319272160; 3319272160; 3319272144; 9783319272146
• Related ISBNs: 9783319272146
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed May 18, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.