DNA Methylation Protocols. (2017)

• Record Type: Book
• Further Information: Note: Jörg Tost.
• Editors: Tost, Jörg
• Contents: Preface…Table of Contents…Contributing Authors…Part I Introduction1. A Summary of the Biological Processes, Disease-Associated Changes and Clinical Applications of DNA MethylationGitte Brinch Andersen and Jörg Tost2. Considerations for Design and Analysis of DNA Methylation StudiesKarin B. Michels and Alexandra M. BinderPart II Global DNA Methylation Levels3. Quantification of Global DNA Methylation Levels by Mass SpectrometryAgustin F. Fernandez, Luis Valledor, Fernando Vallejo, Maria Jesús Cañal, and Mario F. Fraga4. Antibody Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections and Mammaliam EmbryosNathalie Beaujean, Juliette Salvaing, Nur Annies Abd Hadi, and Sari PenningsPart III Genome-Wide DNA Methylation Analysis5. Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq ProtocolChristian Daviaud, Victor Renault, Florence Mauger, Jean-François Deleuze, and Jörg Tost 6. Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite SequencingDieter Weichenhan, Qi Wang, Andrew Adey, Stephan Wolf, Jay Shendure, Roland Eils, and Christoph Plass7. Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite SequencingFumihito Miura and Takashi Ito8. Multiplexed Reduced Representation Bisulfite Sequencing with Magnetic Bead Fragment Size SelectionWilliam P. Accomando and Karin B. Michels9. Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging ApproachJulian R. Peat and Sébastien A. Smallwood10. Methyl-CpG Binding Domain Sequencing: MBD-seqKarolina A. Aberg, Robin F. Chan, Linying Xie, Andrey Shabalin, and Edwin J.C.G. van den Oord11. The HELP-Based AssaysJohn M. Greally12. Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seqXiaoyun Xing, Bo Zhang, Daofeng Li, and Ting Wang13. Digital Restriction Enzyme Analysis of Methylation (DREAM)Jaroslav Jelinek, Justin T. Lee, Matteo Cesaroni, Jozef Madzo, Shoudan Liang, Yue Lu, and Jean-Pierre J. Issa14. Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)Fides D. Lay, Theresa K. Kelly, and Peter A. Jones15. Bisulfite Sequencing of Chromatin Immunoprecipitated DNA (BisChIP-seq)Clare Stirzaker, Jenny Z. Song, Aaron L. Statham, and Susan J. Clarkdiv>16. A Guide to Illumina BeadChip Data AnalysisMichael C. Wu and Pei-Fen Kuan Part IV Highly Multiplexed Target Regions17. Microdroplet PCR for Highly-Multiplexed Targeted Bisulfite SequencingH. Kiyomi Komori, Sarah A. LaMere, Traver Hart, Steven R. Head, Ali Torkamani, and Daniel R. Salomon 18. Multiplexed DNA Methylation Analysis of Target Regions Using Microfluidics (Fluidigm)Martyna Adamowicz-Brice, Klio Maratou, and Timothy J. Aitman19. Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock ProbesDinh Diep, Nongluk Plongthonkum, and Kun Zhang20. Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment SystemJennifer Wendt, Heidi Rosenbaum, Todd Richmond, Jeffrey A. Jeddeloh, and Daniel Burgess21. Multiplexed and Sensitive DNA Methylation Testing Using Methylation-Sensitive Restriction Enzymes « MSRE-qPCR »Gabriel Beikirch, Walter Pulverer, Manuela Hofner, Christa Noehammer, and Andreas WeinhaeuselPart V Locus-Specific DNA Methylation Analysis 22. Quantitative DNA Methylation Analysis at Single-Nucleotide Resolution by PyrosequencingFlorence Busato, Emelyne Dejeux, Hafida El abdalaoui, Ivo G. Gut, and Jörg Tost23. Methylation-Specific PCRJoão Ramalho-Carvalho, Rui Henrique, and Carmen Jerónimo 24. Quantitation of DNA Methylation by Quantitative Multiplex Methylation-Specific PCR (QM-MSP)Mary Jo Fackler and Saraswati Sukumar25. MethyLight and Digital MethyLightMihaela Campan, Daniel J. Weisenberger, Bin Trinh, and Peter W. Laird26. Quantitative Region-Specific DNA Methylation Analysis by the EPITYPERTM TechnologySonja Kunze27. Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA) Cathy B. Moelans, Lilit Atanesyan, Suivi P. Savola, and Paul J. van Diest28. Methylation - Sensitive - High Resolution Melting (MS-HRM)Dianna Hussmann and Lise Lotte Hansen29. Hairpin Bisulfite Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual ChromosomesPascal Giehr and Jörn Walter30. Helper-Dependent Chain Reaction (HDCR) for Selective Amplification of Methylated DNA SequencesSusan M. Mitchell, Keith N. Rand, Zheng-Zhou Xu, Thu Ho, Glenn S. Brown, Jason P. Ross, and Peter L. MolloyPart VI DNA Methylation Analysis of Specific Biological Samples31. DNA Methylation Analysis from Blood Spots: Increasing Yield and Quality for Genome-Wide and Locus-Specific Methylation AnalysisAkram Ghantous, Hector Hernandez-Vargas, and Zdenko Herceg32. DNA Methylation Analysis of Free-Circulating DNA in Body FluidsMaria Jung, Glen Kristiansen, and Dimo DietrichPart VII Hydroxymethylation33. Tet-Assisted Bisulfite Sequencing (TAB-seq)Miao Yu, Dali Han, Gary C, Won, and Chuan He34. Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq)Kristina Kirschner, Felix Krueger, Anthony R. Green, and Tamir Chandra35. Affinity-Based Enrichment Techniques for the Genome-Wide Analysis of 5-HydroxymethylcytosineJohn P. Thomson and Richard R. Meehan.
• Edition: Third edition
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2018
• Extent: 1 online resource (704 pages)
• Subjects: Medicine; Human genetics; Medical -- Genetics; Medical genetics
• Languages: English
• ISBNs: 9781493974818
• Related ISBNs: 9781493974795
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.