Stem cell heterogeneity : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Kursad Turksen.
• Editors: Turksen, Kursad
• Contents: The Heterogeneity of Stem Cells: A Brief Overview -- Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells -- An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells -- Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter -- Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells -- Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells -- Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs) -- Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice -- Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing -- Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity -- Visualizing the Functional Heterogeneity of Muscle Stem Cells -- Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies -- Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells -- Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering -- In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry -- Aerosol-Based Cell Therapy for Treatment of Lung Diseases -- Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro -- Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro -- Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro -- Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method -- Automated Cell-Based Quantitation of 8-OHdG Damage -- CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis -- Reporter Systems to Study Cancer Stem Cells -- Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth -- Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon -- Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors -- Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xiii, 390 pages), illustrations (some color)
• Subjects: 616.02/774; Life sciences; Stem cells -- Laboratory manuals; Stem cells; Stem Cells; Cell Differentiation; Cell Lineage; Genetic Heterogeneity; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493965502; 1493965506
• Related ISBNs: 9781493965496; 1493965492
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.