CCN proteins : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Masaharu Takigawa.
• Other Names: Takigawa, Masaharu
• Contents: The CCN Proteins: An Overview -- Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues -- Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols -- Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells -- Western Blotting Analysis of CCN Proteins -- Immunohistochemical Analysis of CCN Proteins -- Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis -- Production of Recombinant CCN2 Protein in Escherichia. Coli -- Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis -- Production Of Recombinant CCN2 Protein By Mammalian Cells -- In Vitro Transfection with and Expression of CCN Family of Genes -- Preparation of Module Specific Antibodies against CCN2 -- ELISA of CCN Proteins in Body Fluids Including Serum And Plasma -- Analysis of Signaling Pathways Activated by CCN Proteins -- Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System -- Protocols For Screening Peptide Motifs Binding To CCN Proteins -- Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR) -- Promoter Analysis of CCN Genes -- Analysis Of Posttranscriptional Regulation Of CCN Genes -- Protein Imaging of CCN2 and CCN3 in Living Cells -- Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins -- Protocol for Measuring Angiogenic Activities of CCN Proteins -- Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins -- Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies -- In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins -- Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress -- The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice -- Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters -- Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches -- Construction and Analysis of an Allelic Series of CCN1 Knockin Mice -- Production and Analysis of Conditional KO Mice of CCN2 in Kidney -- Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice -- Analysis of Transcytosis of CCN2 by Chondrocytes -- CCN2 in Skin Fibrosis -- Studying the CCN Proteins in Fibrosis -- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases -- Kidney Fibrosis -- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases -- Liver Fibrosis -- Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis -- TGF-?1 snd CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells -- CCN Detection of Cancer Tissues by Immunohistochemistry Staining -- Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique -- Analysis of Pathological Activities of CCN Proteins in Bone Metastasis -- Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy -- Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System -- An Analysis of Pathological Activities of CCN Proteins in Joint Disorders{u2212}Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells{u2212} -- Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies. .
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2017
• Extent: 1 online resource (xv, 576 pages), illustrations (some color)
• Subjects: 572/.65; Life sciences; Cysteine -- Laboratory manuals; Cell interaction -- Laboratory manuals; Cell interaction; Cysteine; CCN Intercellular Signaling Proteins; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Laboratory manuals; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493964307; 1493964305
• Related ISBNs: 9781493964284; 1493964283
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from pdf of title page (SpringerProtocols, viewed October 14, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.