Enhancer RNAs : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Ulf Andersson Ørom.
• Editors: Ørom, Ulf Andersson
• Contents: Cellular Fractionation and Isolation of Chromatin-associated RNA.-Knockdown of Nuclear-located Enhancer RNAs and Long ncRNAs using Locked Nucleic Acid GapmeRs.-Visualization of Enhancer-derived Non-coding RNA.-UV-RNA Immunoprecipitation (UV-RIP) Protocol in Neurons.-Mapping Long non-coding RNA Chromatin Occupancy using Capture Hybridization Analysis of RNA Targets (CHART).- Detecting long-range Enhancer-promoter Interactions by Quantitative Chromosome Conformation Capture.-Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET).-Identification of Transcribed Enhancers by Genome-wide Chromatin Immunoprecipitation Sequencing.-Global Run-On sequencing (GRO-seq).-Computational Approaches for Mining GRO-seq Data to Identify and Characterize Active Enhancers.-Evaluating the Stability of mRNAs and Non-coding RNAs.-A Novel Method to Quantify RNA-protein Interactions in situ using FMTRIP and Proximity Ligation.-In silico Promoter Recognition from deepCAGE data.-Bioinformatics Pipeline for Transcriptome Sequencing Analysis.-CRISPR/Cas-9 Genome Editing in Embryonic Stem Cells.-Targeted Gene Activation Using RNA-Guided Nucleases.
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Copyright Date: 2017
• Extent: 1 online resource (252 pages)
• Subjects: 572.88; Medicine; Non-coding RNA; Human genetics; Medical -- Genetics; Medical genetics
• Languages: English
• ISBNs: 9781493940356
• Related ISBNs: 9781493940332
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.