Oogenesis : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Ioannis P. Nezis.
• Editors: Nezis, Ioannis P
• Contents: Histological Analysis of the Arabidopsis Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference Contrast Light Microscopy -- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans -- Antibody Staining In Drosophila Germaria -- In Vitro<Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method -- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cells in Drosophila Melanogaster -- Analysis of Phagocytosis in the Drosophila Ovary -- Immuno-Gold Labeling Of Drosophila Follicles For Transmission Electron Microscopy -- Preparation of Drosophila Follicles for Transmission Electron Microscopy -- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles -- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling, Annotating, and Analysing your Data -- Live Imaging of Centriole Dynamics by Fluorescently-Tagged Proteins un Starfish Oocyte Meiosis -- High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes -- Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes -- RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes -- Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis -- Quantitative Microinjection of Morpholino Antisense Oligonucleotides into Mouse Oocytes to Examine Gene Function in Meiosis-I -- Monitoring Calcium Oscillations in Fertilized Mouse Eggs -- Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes -- Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence Activated Cell Sorting -- Immunohistochemistry of Paraffin Sections from Mouse Ovaries.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (x, 277 pages), illustrations
• Subjects: 571.8/45; Life sciences; Oogenesis -- Laboratory manuals; Oogenesis; Oogenesis -- physiology; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493937950; 1493937952
• Related ISBNs: 9781493937936; 1493937936
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.