Optical tweezers : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Arne Gennerich.
• Editors: Gennerich, Arne
• Contents: Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers": Dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the clpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (xii, 555 pages), illustrations (some color)
• Subjects: 621.3815/2; Chemistry; Optical tweezers -- Laboratory manuals; Optical tweezers; Optical Tweezers; Optical Imaging -- methods; Micromanipulation -- methods; Biotechnology; Science -- Biotechnology; Biotechnology; Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493964215; 1493964216
• Related ISBNs: 9781493964192; 1493964194
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed December 1, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.