Cis/transgene optimization : systematic discovery of novel gene expression elements using bioinformatics and computational biology approaches /: systematic discovery of novel gene expression elements using bioinformatics and computational biology approaches. ([2018])

• Record Type: Book
• Further Information: Note: Saeid Kadkhodaei, [and 10 others].
• Authors: Kadkhodaei, Saeid
• Contents: Intro; Preface; Acknowledgements; Contents; Acronyms; 1 Introduction; 1.1 Overview; 1.2 Scaffold/Matrix Attachment Regions (S/MARs); 1.3 Translation Initiation Site (TIS); 1.4 Signal Peptides; 1.5 Gene Optimization; References; 2 Systematic Strategies; 2.1 MARs (Fig. 2.1a); 2.1.1 S/MARs Data Mining, Motif Search, and Comparison in Databases; 2.1.2 Heatmap Cluster and Correlation Plot; 2.1.3 Scatter Plot for Correlation; 2.1.4 Design, Evaluation, and Synthesis of an Artificial MAR Sequence; 2.2 Translation Initiation Site (Fig. 2.1b); 2.2.1 Data Mining of the Microalgae MRNAs in Databases. 2.2.2 In Silico Analysis of the Full-Length mRNAs2.3 Signal Peptide Prediction (Fig. 2.1c); 2.4 Gene Optimization (Fig. 2.1d); 2.5 Microalgae Transformation; References; 3 Outcomes Assessment; 3.1 Scaffold/Matrix Attachment Regions (S/MARs); 3.1.1 Statistical Analysis of the S/MARs Structure; 3.1.2 Significant Motifs; 3.1.3 Intercorrelation Analysis; 3.1.4 Clustering Pattern Analysis; 3.1.5 Motif Mapping; 3.1.6 CpG Islands; 3.2 Translation Initiation Site (TIS); 3.3 Signal Peptide (SP) Prediction; 3.4 Gene Optimization; 3.4.1 Codon Optimization; 3.4.2 mRNA Secondary Structure. 3.5 Transformation3.5.1 Transformation Techniques; References; 4 Conceptual Models; 4.1 Scaffold/Matrix Attachment Regions (S/MARs); 4.2 Translation Initiation Site (TIS); 4.3 Signal Peptide Prediction; 4.4 Gene Optimization; 4.4.1 Codon Optimization; 4.4.2 mRNA Secondary Structure; 4.4.3 Additional Optimization Parameters; 4.5 Transformation; 4.6 Summary; References; Appendix A; Appendix B; Appendix C; Appendix D; Appendix E; Appendix F; Appendix G; Appendix H; Appendix I: The Detailed Methodology of the Transformation Procedure; Appendix J; Appendix K; Appendix L; Appendix M; Appendix N. Appendix OAppendix P; Appendix Q; Appendix R; Appendix S; Appendix T; Appendix U; Appendix V; Index.
• Publisher Details: Cham, Switzerland : Springer
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 660.6/5; Life sciences; Recombinant proteins; Transgenes -- Expression; Genomics; SCIENCE -- Chemistry -- Industrial & Technical; TECHNOLOGY & ENGINEERING -- Chemical & Biochemical; Genomics; Recombinant proteins; Transgenes -- Expression; Science -- Life Sciences -- General; Computers -- Computer Science; Science -- Life Sciences -- Biochemistry; Life sciences: general issues; Proteins; Biology -- Data processing; Biological models; Bioinformatics; Proteomics; Biology, life sciences; Electronic books
• Languages: English
• ISBNs: 9783319903910; 3319903918
• Related ISBNs: 331990390X; 9783319903903
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from digital title page (viewed on July 19, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.