Genetic transformation systems in fungi. Volume 1 ([2014])

• Record Type: Book
• Further Information: Note: Marco A. van den Berg, Karunakaran Maruthachalam, editors.
• Editors: Berg, Marco A. van den; Maruthachalam, Karunakaran
• Contents: Preface; Contents; Contributors; Part I: Introduction; 1: Fungal Transformation: From Protoplasts to Targeted Recombination Systems; 1.1 Introduction; 1.1.1 A Historical Perspective of Transformation of Fungi; 1.2 Early Development of Basic Tools for Transformation; 1.2.1 Protoplasts and Lytic Enzymes; 1.2.2 Osmotic Stabilization and Protoplasts Regeneration; 1.3 Alternative Transformation Procedures That Do Not Require Protoplasts; 1.3.1 Electroporation; 1.3.2 Agrobacterium-Mediated Transformation; 1.3.3 Biolistic Transformation; 1.4 Selective Markers; 1.4.1 Nutritional Markers. 1.4.2 Metabolic Fitness of Auxotrophic Host Strains: Growth and Secondary Metabolites Production1.4.3 Positive Selection Resistance Markers; 1.5 Autonomously Replicating Plasmids: Stability Problems of Gene Libraries; 1.6 Homologous and Nonhomologous Recombination: Integration of Exogenous Genes; 1.6.1 Targeted Monocopy Integrations at Specific Loci; 1.6.2 The "Two Markers" Selection Strategy for Gene Disruption; 1.6.3 The Nonhomologous End-Joining Mechanism; 1.6.4 Metabolic Fitness of NHEJ-ƯDeficient Mutants; 1.7 Future Outlook; References. 2.3.1.1 Cell Type and Growth Phase2.3.1.2 Growth Conditions; 2.3.1.3 Optimal Enzyme Combination for Lysing the Cell Wall; 2.3.1.4 Stabilizing Protoplasts During and After Removal of the Cell Wall; 2.3.1.5 Incubation Time and Temperature for Lysing the Cell Wall; 2.3.1.6 Evaluation and Storage of Protoplasts; 2.3.1.7 Uptake of DNA/Transformation; 2.3.1.8 Incubation Time and Medium for Protoplast Regeneration; 2.4 Concluding Remarks; References; 3: Trichoderma Transformation Methods; 3.1 General Methods; 3.2 Detailed Procedure Description. 3.2.1 Transformation of Trichoderma Mediated by Protoplasts3.2.1.1 Growth Conditions and Protoplasts Formation; 3.2.1.2 Protoplasts Transformation; 3.2.2 Transformation of Trichoderma Mediated by A. tumefaciens; 3.3 Alternative Trichoderma Transformation Procedures; 3.3.1 Protoplast Electroporation (S©Łnchez-Torres et al. 1994); 3.3.2 Biolistic (Lorito et al. 1993); 3.3.3 Shock Waves (Maga©ła-Ort©Ưz et al. 2013); 3.4 Notes; References; 4: Transformation of Mucor circinelloides f. lusitanicus Protoplasts; 4.1 Introduction; 4.2 Materials; 4.3 Methods; 4.3.1 M. circinelloides Cell-Wall Isolation.
• Publisher Details: Cham : Springer
• Publication Date: 2014
• Copyright Date: 2015
• Extent: 1 online resource (xv, 318 pages), illustrations (some color)
• Subjects: 579/.135; Life sciences; Fungi -- Genetics; SCIENCE -- Life Sciences -- Biology; SCIENCE -- Life Sciences -- Microbiology; Fungi -- Genetics; Science -- Life Sciences -- Botany; Science -- Life Sciences -- General; Botany & plant sciences; Plant pathology & diseases; Plant reproduction & propagation; Life sciences: general issues; Developmental biology; Plant genetics; Plant diseases; Plant breeding; Biological models; Science -- Life Sciences -- Biology -- Developmental Biology; Developmental biology; Electronic books
• Languages: English
• ISBNs: 9783319101422; 3319101420; 3319101412; 9783319101415
• Related ISBNs: 9783319101415
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed November 21, 2014).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.