Epitope mapping protocols. (2018)

• Record Type: Book
• Editors: Rockberg, Johan; Nilvebrant, Johan
• Contents: Preface...Table of Contents...Contributing Authors...1. An Introduction to Epitope MappingJohan Nilvebrant and Johan RockbergPart I Whole Antigen Methods2. Epitope Mapping of Antibody-Antigen Interactions with X-Ray CrystallographyMoeko Toride and Cory L. Brooks3. Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical ConsiderationsLuca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, and Luca Varani4. Label-Free Cell-Based Assay for Characterization of Biomolecules and ReceptorsDiluka Peiris and Lars M. Jørgensen5. Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-LinkersThomas Huber and Thomas P. Sakmar6. Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep SequencingTariq Ahmad Najar, Shruti Khare, and Raghavan Varadarajan7. Epitope Mapping Using Yeast Display and Next Generation SequencingThomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton, Dilduz Telman, Lora Zhao, Wai Ling Cheung, Jan Berka, Wenwu Zhai, Pavel Strop, Jaume Pons, Arvind Rajpal, and Javier Chaparro-RiggersPart II Protein Domain and Fragment-Based Methods8. B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent AssayThiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, and Michael W. Heuzenroeder9. Array-In-Well Epitope Mapping of Phage-Displayed AntibodiesUrpo Lamminmäki, Gaurav Batra, and Petri Saviranta10. Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment LibrariesAnna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg11. Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded DomainsAnna-Luisa Volk and Johan RockbergPart III Real-Time Based Methods12. Kinetic Analysis and Epitope Binning Using Surface Plasmon ResonanceJohan Nilvebrant13. Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer InterferometryLisette Bronswijk-DeddensPart IV Antibody Specificity and T-Cell Prediction14. Antibody Specificity Profiling Using Protein MicroarraysPedro Ramos-López, José Irizarry, Ignacio Pino, and Seth Blackshaw15. High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target BindingRonald Sjöberg, Eni Andersson, Cecilia Hellström, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson, and Burcu Ayoglu16. Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope MappingBurcu Ayoglu, Peter Nilsson, and Jochen M. Schwenk17. Antibody Biomarker Discovery Via In Vitro Evolution of PeptidesJohn T. Ballew, Jack R. Reifert, and Patrick S. Daugherty18. Bioinformatics Tools for the Prediction of T Cell EpitopesMassimo Andreatta and Morten Nielsen.
• Edition: Third edition
• Publisher Details: New York : Humana Press
• Publication Date: 2018
• Copyright Date: 2018
• Extent: 1 online resource (284 pages)
• Subjects: 616.0792; Medicine; Antigenic determinants; Immunology; Medical -- Immunology; Immunology
• Languages: English
• ISBNs: 9781493978410
• Related ISBNs: 9781493978397
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.