High content screening : a powerful approach to systems cell biology and phenotypic drug discovery /: a powerful approach to systems cell biology and phenotypic drug discovery. ([2018])

• Record Type: Book
• Further Information: Note: Edited by Paul A. Johnston, Oscar J. Trask.
• Editors: Johnston, Paul A; Trask, Oscar J
• Contents: Part I: Reagents and Kits for Measuring Cells and their Phenotypes 1. Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays Roy Edward 2. General Staining and Segmentation Procedures for High Content Imaging and Analysis Kevin M Chambers, Bhaskar S Mandavilli, Nick J Dolman and Michael S Janes 3. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis Bhaskar S Mandavilli, Robert J Aggeler, and Kevin Chambers 4. Cell-based High Content Analysis of Cell Proliferation and Apoptosis Bhaskar S Mandavilli, Michelle Yann and Scott Clarke 5. Tools to Measure Autophagy using High Content Imaging and Analysis Nick J Dolman, Brent A. Samson, Kevin M. Chambers, Michael S Janes, and Bhaskar S Mandavilli Part II: Selecting Microtiter Plates, Identify Imaging Artifacts, Rnai Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS 6. Guidelines for Microplate Selection in High Content Imaging O.J. Trask 7. Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler Mark-Anthony Bray and Anne E. Carpenter 8. High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening Steven A. Haney 9. Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays Karol Kozak, Bernt Rinn, Oliver Leven, and M. Emmenlauer 10. Live-Cell High Content Screening in Drug Development Milan Esner, Felix Meyenhofer, and Marc Bickle 11. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O'Connell, Martyn Banks, and Andrea Weston Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis 12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells Verena Fetz, Roland H. Stauber, and Shirley K. Knauer 13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston 14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis 15. Single Cell and Population Level Analysis of HCA Data David Novo, Kaya Ghosh, and Sean Burke 16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens Judith Wardwell-Swanson and Yanhua Hu Section IV: Phenotypic Models – Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos 17. High Content Screening of Mammalian Primary Cortical Neurons Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon 18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications Joshua A. Harrill 19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays Oksana Sirenko and Evan F. Cromwell 20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, and Paul A. Johnston 21. An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, and Mark Uhlik 22. High-throughput Automated Chemical Screens in Zebrafish Manush Saydmohammed and Michael Tsang.
• Edition: Second edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2018
• Copyright Date: 2018
• Extent: 1 online resource (xiii, 397 pages), illustrations (some color)
• Subjects: 570.72; Chemistry; Biological systems -- Research -- Methodology; Computational biology; Drug development -- Computer simulation; Computational biology; Biotechnology; Science -- Biotechnology; Biotechnology; Electronic books
• Languages: English
• ISBNs: 9781493973576; 1493973576
• Related ISBNs: 9781493973552; 149397355X
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed November 1, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.