Mammalian synthetic promoters. (2017)

• Record Type: Book
• Further Information: Note: Edited by David Gould.
• Editors: Gould, David
• Contents: Initial Considerations before Designing a Promoter Construct -- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay -- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter -- Secreted Reporters for Monitoring Multiple Promoter Function -- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo -- Monitoring Promoter Activity by Flow Cytometry -- Functional Screening of Core Promoter Activity -- Bioinformatically Informed Design of Synthetic Mammalian Promoters -- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication -- Constructing Strong Cell-Type-Specific Promoters through Informed Design -- PCR Assembly of Synthetic Promoters -- The Tetracycline Responsive System -- Light-responsive Promoters -- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation -- Promoter Activation with Electromagnetism -- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment -- A Modular Approach to Building Complex Synthetic Circuits -- Computational Sequence Design with R2oDNA Designer -- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells. .
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Extent: 1 online resource (xi, 277 pages), illustrations (some color)
• Subjects: 572.8/6; Life sciences; Promoters (Genetics); Bacteriology; Promoters (Genetics); Science -- Life Sciences -- Bacteriology; Bacteriology (non-medical); Promoter Regions, Genetic; Electronic books
• Languages: English
• ISBNs: 9781493972234; 1493972235
• Related ISBNs: 9781493972210; 1493972219
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed August 17, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.