Rho GTPases : Methods and Protocols /: Methods and Protocols. (2018)

• Record Type: Book
• Further Information: Note: Francisco Rivero.
• Editors: Rivero, Francisco
• Contents: Part I: Computational Modeling and Imaging 1. Computational Modeling of the Dynamics of Spatiotemporal Rho GTPase Signaling: A Systematic Review Shabnam Khatibi, Karina Islas Rios, and Lan K. Nguyen 2. Uncovering Bistability in the Rac1/RhoA Signaling Network through Integrating Computational Modeling and Experimentation Alex von Kriegsheim and Lan K. Nguyen 3. Modeling Rho GTPase Dynamics Using Boolean Logic Joseph H.R. Hetmanski, Jean-Marc Schwartz, and Patrick T. Caswell 4. EdgeProps: A Computational Platform for Correlative Analysis of Cell Dynamics and Near-Edge Protein Activity Anastasia Zhurikhina, Timothy Qi, Klaus M. Hahn, Timothy C. Elston, and Denis Tsygankov 5. Applying Perturbation Expectation-Maximization to Protein Trajectories of Rho GTPases Peter K. Koo and Simon G.J. Mochrie 6. Parallel High Resolution Imaging of Leukocyte Chemotaxis under Agarose with Rho Family GTPase Biosensors George R.R. Bell, Dean E. Natwick, and Sean R. Collins 7. Characterization of Genetically-Encoded FRET Biosensors for Rho Family GTPases Sara K. Donnelly, Veronika Miskolci, Alice M. Garrastegui, Dianne Cox, and Louis Hodgson 8. Screening for Rho GTPase Modulators in Actin-Dependent Processes Exemplified by Phagocytosis Daniel Schlam, Sergio Grinstein, and Spencer A. Freeman Part II: Biochemistry and High Throughput Methods 9. An In Vitro Kinase Assay to Assess Rac1 Phosphorylation by ERK Daniel Brandwein, Junfeng Tong, Laiji Li, Barbara Ballermann, and Zhixiang Wang 10. Methods to Study Rho GTPases Using Bacterial Toxins Gudula Schmidt 11. Assessment of S-Glutathionylated Rac1 in Cells Using Biotin-Labeled Glutathione Hannah Edenbaum and Jingyan Han 12. High Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay Robert G. Lowery and Meera Kumar 13. Small Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases Peter Simons, Virginie Bondu, Angela Wandinger-Ness, and Tione Buranda Part III: Cell Types and Processes 14. Methods to Study the Roles of Rho GTPases in Platelet Function Francisco Rivero and Simon Calaminus 15. Methods to Investigate the Role of Rho GTPases in Osteoclast Function Anne Morel, Anne Blangy, and Virginie Vives 16. Methods to Study the Role of Cdc42, Rac1 and Rac2 in B Cell Cytoskeletal Responses Natalija Gerasimcik, Lisa S. Westerberg, and Eva Severinson 17. In Vivo Rescue Assay of RhoA Deficient Hematopoietic Stem and Progenitor Cells Xiongwei Cai, Xuan Zhou, and Yi Zheng 18. Cell Polarity and Division Symmetry Analyses in Transformed Blood Cells Benjamin Mizukawa, Eric O'Brien, James C. Mulloy, and Yi Zheng 19. C3 Transferase Gene Therapy for Continuous RhoA Inhibition Angel J. Santiago-Lopez, Claire-Anne Gutekunst, and Robert E. Gross 20. A Practical Protocol for the Conditional Depletion of Rho Isoforms in Human Embryonic Stem Cells Masatoshi Ohgushi 21. Methods to Study the Roles of Rho GTPases in Dendritic Tree Complexity Jana Schulz and Stefan Schumacher 22. Assessing the Roles of Rho GTPases in Cell DNA Repair by the Nucleotide Excision Repair Pathway Lilian C. Russo, Pault Y. Minaya, Luiz E. Silva, and Fábio L. Forti 23. Measuring the Contributions of the Rho Pathway to the DNA Damage Response in Tumor Epithelial Cells Yuli T. Magalhães, Jessica O. Farias, Lucas F. Monteiro, and Fábio L. Forti Part IV: Rho GTPases in Non-Mammalian Model Organisms 24. Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish Nicholas J. Hanovice, Emily McMains, and Jeffrey M. Gross 25. Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum Maja Marinović, Huajiang Xiong, Francisco Rivero, and Igor Weber 26. Reconstruction of ROP GTPase Domains on the Plasma Membrane in Tobacco Leaves Yoshihisa Oda, Yoshinobu Nagashima, and Hiroo Fukuda 27. Analyzing Rho GTPase-Dependent Processes during Cell Polarization in Brown Algae Whitney Hable.
• Edition: Second edition
• Publisher Details: New York : Humana Press
• Publication Date: 2018
• Copyright Date: 2018
• Extent: 1 online resource (419 pages)
• Subjects: Life sciences; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins
• Languages: English
• ISBNs: 9781493986125
• Related ISBNs: 9781493986118
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.