Histones : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Benoit Guillemette, Luc R. Gaudreau.
• Editors: Guillemette, Benoit; Gaudreau, Luc
• Contents: In Vitro Assembly of Nucleosomes For Binding/Remodeling Assays -- An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays -- Histone Purification from Saccharomyces Cerevisiae -- Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, Hplc-Purified Histones -- Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples -- Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies -- Production and Purification of Antibodies against Histone Modifications -- Immunofluorescence of Histone Proteins -- Acid-Urea Gel Electrophoresis and Western Blotting Of Histones -- Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast -- A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae -- High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology -- Physarum Polycephalum for Studying the Function Of Histone Modifications In Vivo -- A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins. .
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (x, 279 pages), illustrations (some color)
• Subjects: 572/.66; Life sciences; Histones -- Laboratory manuals; Histones; Histones; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493966301; 1493966308; 1493966286; 9781493966288
• Related ISBNs: 9781493966288; 9781493933601
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed December 1, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.