Oat : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Sebastian Gasparis, Department of Functional Genomics, Plant Breeding & Acclimatization Inst., National Research Institute, Blonie, Poland.
• Editors: Gasparis, Sebastian
• Contents: Preface… Table of Contents…Contributing Authors…Part I Cytological Methods1.Fluorescence In Situ Hybridization in OatEva Wegel2.Oat Doubled Haploids Following Maize PollinationPhilip A. Davies and Parminder K. Sidhu3.Wide Hybridization between Oat and Pearl MilletTakayoshi Ishii4.Protocol for Producing Synthetic Polyploid OatsToshinobu MorikawaPart II In Vitro Cultures and Genetic Transformation5.Manipulation of Oat Protoplasts for Transient Expression AssaysRobyn Roberts, Jincan Zhang, Nicole Mihelish, Danielle Savino, Aurélie M. Rakotondrafara6.Oat Anther Culture and Use of DH-Lines for Genetic MappingElina Kiviharju, Sirpa Moisander, and Pirjo Tanhuanpää7.Agrobacterium-Mediated Transformation of Leaf Base SegmentsSebastian GasparisPart III Quality Assessment8.Chromatographic Methods to Evaluate Nutritional Quality in OatGracia Montilla-Bascón, Corey D. Broeckling, Owen A. Hoekenga, Elena Prats, Mark Sorrells, and Julia Isidro-Sánchez9.Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass SpectrometryVeronica M.T. Lattanzio and Michelangelo Pascale10.Multiplex Dipstick Immunoassay for Semi-Quantitative Determination of Fusarium Mycotoxins in OatVeronica M.T. Lattanzio and Noan Nivarlet11.Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in OatSusanna Oswald, Richard Dietrich, Erwin Märtlbauer, Reinhard Niessner, and Dietmar KnoppPart IV Genomic Studies12. M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat GermplasmAgnieszka Onyśk and Maja Boczkowska13.Genotyping-By-Sequencing and its Application to Oat Genomic ResearchYong-Bi Fu and Mo-Hua Yang14. Genome-Wide Association Analysis using RJulio Isidro Sánchez, Deniz Akdemir, and Gracia Montilla-Bascón15.de novo Transcriptome Assembly in Polyploid SpeciesJuan J. Gutierrez-Gonzalez and David F. GarvinPart V Proteomics Methods16.Isolation of Oat (Avena sativa L.) Total Proteins and their Prolamin Fractions for 2-D ElectrophoresisDorota Nałęcz, Marta Dziuba, and Iwona Szerszunowicz17. 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and their Prolamin FractionsDorota Nałęcz, Iwona Szerszunowicz, Marta Dziuba, and Piotr Minkiewicz18. Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques used in the Strategy for the Identification of Oat Proteins after 2-DEIwona Szerszunowicz, Dorota Nałęcz, and Marta Dziuba.
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (272 pages)
• Subjects: 633.1/3; Life sciences; Botany; Avena -- genetics; Genomics -- methods; Science -- Life Sciences -- Botany; Botany & plant sciences; Laboratory Manuals
• Languages: English
• ISBNs: 9781493966820
• Related ISBNs: 9781493966806
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.