Peroxisomes : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Uniform Title: Peroxisomes (Schrader)
• Further Information: Note: Edited by Michael Schrader, College of Life and Environmental Sciences, Biosciences, University of Exeter, Exeter, UK.
• Editors: (Professor of cell biology), Schrader, Michael
• Contents: 1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation Andreas Manner and Markus Islinger 2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes Miriam J. Schönenberger and Werner J. Kovacs 3. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo 4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae Tobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann 5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp 6. Analysis of Plasmalogen Synthesis in Cultured Cells Masanori Honsho and Yukio Fujiki< 7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies Janet Koster and Hans R. Waterham 8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells Tina A. Schrader and Michael Schrader 9. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms 10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3, 3'-diaminobenzidine H. Dariush Fahimi 11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins Karina Mildner and Dagmar Zeuschner 12. Detection and Immunolabelling of Peroxisomal Proteins Tina Schrader, Markus Islinger, and Michael Schrader 13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis Sofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster 14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes Celien Lismont, Paul A. Walton, and Marc Fransen 15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress Marc Fransen and Chantal Brees 16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed Luis F. Godinho, and Michael Schrader 17. In Cellulo Approaches to Study Peroxisomal Protein Import – Yeast Immunofluorescence Microscopy Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann 18. Blue Native Page: Applications to Study Peroxisome Biogenesis Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki 19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes Yuqiong Liu, Masanori Honsho, and Yukio Fujiki 20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki 21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells Judith Buentzel, and Sven Thoms 22. Detection of Ubiquitinated Peroxisomal Proteins in Yeast Natasha Danda and Chris Williams 23. Assessing Pexophagy in Mammalian Cells Shun-ichi Yamashita and Yukio Fujiki 24. Experimental Systems to Study Yeast Pexophagy Shun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, and Yukio Fujiki 25. Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells Thomas Nury, Imen Helali, El Mostafa Karym, Meryam Debbabi, Flore Geillon, Catherine Gondcaille, Doriane Trompier, Amina Najid, Sébastien Terreau, Maryem Bezine, Amira Zarrouk, Anne Vejux, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Stéphane Savary, and Gérard Lizard 26. Study of Peroxisomal Protein Phosphorylation by Functional Proteomics Andreas Schummer, Sven Fischer, Silke Oeljeklaus, and Bettina Warscheid 27. Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings Björn Hielscher, Lennart Charton, Tabea Mettler-Altmann, and Nicole Linka 28. Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy Noa Dahan, Maya Schuldiner, and Einat Zalckvar 29. Generation of Peroxisome-Deficient Somatic Animal Cell Mutants Kanji Okumoto and Yukio Fujiki 30. Clinical and Laboratory Diagnosis of Peroxisomal Disorders Ronald J.A. Wanders, Femke C.C. Klouwer, Sacha Ferdinandusse, Hans R. Waterham, and Bwee Tien Poll-Thé.
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (347 pages)
• Subjects: 571.6/55; Life sciences; Peroxisomes -- Laboratory manuals; Peroxisomes; Peroxisomes; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Laboratory Manuals; Laboratory manuals
• Languages: English
• ISBNs: 9781493969371
• Related ISBNs: 9781493969357
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.