RNAi and small regulatory RNAs in stem cells : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Baohong Zhang.
• Editors: Dr, Zhang, Baohong
• Contents: Part I: Introduction 1. RNAi Technique in Stem Cell Research: Current Status and Future Perspectives Gang-Ming Zou 2. RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells Alexander K. Murashov Part II: RNA Interference 3. Forward RNAi Screens in Human Hematopoietic Stem Cells Roman Galeev, Christine Karlsson, Aurélie Baudet, and Jonas Larsson 4. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, and Tieqiao Wen 5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Löhr 6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells Xiaoxing Cheng and Rui Jian 7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, and Matthieu Gérard 8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8 Takayuki Manabe and Akio Wanaka 9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells Melanie I. Trombly and Xiaozhong Wang 10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu 11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang 12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann 13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables 14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation Veit Riechmann 15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans Myon-Hee Lee and Dong Suk Yoon Part III: MicroRNAs 16. Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology Gaoying Ren and Detlev Boison 17. Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs Jianlong Wang 18. Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation Xiaoping Pan, Alexander K. Murashov, Edmund J. Stellwag, and Baohong Zhang Part IV: CRISPR-Cas9 and Genome Editing 19. Genetic Tools for Self-Organizing Culture of Mouse Embryonic Stem Cells Via Small Regulatory RNA-Mediated Technologies, CRISPR/Cas9 and Inducible RNAi Nozomu Takata, Eriko Sakakura, Tetsushi Sakuma, and Takashi Yamamoto 20. CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells Yinghua Wang, Yifu Ding, and Jinsong Li.
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (311 pages)
• Subjects: 572.88; Life sciences; Small interfering RNA; RNA; Stem cells; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology)
• Languages: English
• ISBNs: 9781493971084
• Related ISBNs: 9781493971060
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.