The bacterial nucleoid : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Olivier Espéli.
• Editors: Espéli, Olivier
• Contents: Preface… Table of Contents… Contributing Authors… Part I Molecular Genetic Methods to Study Bacterial Nucleoid 1. Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli Jack A. Bryant and David J. Lee 2. Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes N. Patrick Higgins 3. Revealing Sister Chromatid Interactions with the loxP/Cre Recombination Assay Elise Vickridge, Charlène Planchenault, and Olivier Espéli 4. Transposon Insertion Site Sequencing for Synthetic Lethal Screening Yoshiharu Yamaichi and Tobias Dörr Part II Study of bacterial nucleoid with whole genome analysis method 5. WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites Mickaël Poidevin, Elisa Galli, Yoshiharu Yamaichi, and François-Xavier Barre < 6. High Resolution Chromatin Immunoprecipitation: ChIP-Sequencing Roxanne E. Diaz, Aurore Sanchez, Véronique Anton Le Berre, and Jean-Yves Bouet 7. Generation and Analysis of Chromosomal Contact Maps of Bacteria Martial Marbouty and Romain Koszul 8. Nucleoid Associated Proteins: Genome Level Occupancy and Expression Analysis Parul Singh and Aswin Sai Narain Seshasayee Part III Molecular Biology Methods to Study Nucleoid Structuring Factors 9. Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins Sanja Mehandziska, Mircea A. Petrescu, and Georgi Muskhelishvili 10. A chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions Larissa Wilhelm and Stephan Gruber 11. Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins Ramon A. van der Valk, Niels Laurens, and Remus T. Dame 12. Biochemical Analysis of Bacterial Condensins Zoya M. Petrushenko and Valentin V. Rybenkov 13. Exploring Condensins with Magnetic Tweezers Rupa Sarkar and Valentin V. Rybenkov 14. Applications of Magnetic Tweezers to Studies of NAPs Ricksen S. Winardhi and Jie Yan 15. A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data Damien Larivière, Rodrigo Galindo-Murillo, Eric Fourmentin, Samuel Hornus, Bruno Lévy, Julie Papillon, Jean-François Ménétret, and Valérie Lamour Part IV Imaging Bacterial Nucleoid 16. Multi-Locus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization Bryan J. Visser, Mohan C. Joshi, and David Bates 17. Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage Gaëlle Demarre, Victoria Prudent, and Olivier Espéli 18. Measuring ivo Protein Dynamics throughout the Cell Cycle using Microfluidics Roy de Leeuw, Peter Brazda, M. Charl Moolman, J.W.J. Kerssemakers, Belen Solano, and Nynke H. Dekker 19. Imaging of Bacterial Chromosome Organization by 3D Super-Resolution Microscopy Antoine Le Gall, Diego I. Cattoni, and Marcelo Nollmann 20. Sequential Super-Resolution Imaging of Bacterial Regulatory Proteins, the Nucleoid and the Cell Membrane in Single, Fixed E. coli Cells Christoph Spahn, Mathilda Glaesmann, Yunfeng Gao, Yong Hwee Foo, Marko Lampe, Linda J. Kenney, and Mike Heilemann 21. Procedures for Model-Guided Data Analysis of Chromosomal Loci Dynamics at Short Time Scales Marco Gherardi and Marco Cosentino Lagomarsino Part V Biophysics of the Bacterial Nucleoid 22. Isolation and Characterization of Bacterial Nucleoids in Microuidic Devices James Pelletier and Suckjoon Jun 23. Modeling Bacterial DNA: Simulation of Self-Avoiding Supercoiled Worm-Like Chains Including Structural Transitions of the Helix Thibaut Lepage and Ivan Junier 24. Molecular Dynamics Simulation of Supercoiled, Knotted and Catenated DNA Molecules, Including Modeling of Action of DNA Gyrase Dusan Racko, Fabrizio Benedetti, Julien Dorier, Yannis Burnier, and Andrzej Stasiak.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Extent: 1 online resource (xiv, 374 pages), illustrations (some color)
• Subjects: 579.3/135; Life sciences; Bacterial genetics -- Laboratory manuals; Bacterial genetics; Bacteria -- genetics; Microbiology; Science -- Life Sciences -- Biology -- Microbiology; Microbiology (non-medical); Electronic books; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493970988; 1493970984
• Related ISBNs: 9781493970971; 1493970976
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed August 31, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.