Heterologous protein production in CHO cells : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Paula Meleady.
• Editors: Meleady, Paula
• Contents: Strategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells -- Glycoengineering of CHO Cells to Improve Product Quality -- Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension -- Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression -- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells -- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors -- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells -- Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture -- Selection of High-Producing Clones using FACS for CHO Cell Line Development -- The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity -- A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data -- Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells -- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells -- Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture -- Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells -- Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Extent: 1 online resource (x, 252 pages), illustrations (some color)
• Subjects: 660.6/3; Life sciences; Recombinant proteins -- Laboratory manuals; Cell culture -- Laboratory manuals; Pharmaceutical biotechnology -- Laboratory manuals; Cell culture; Pharmaceutical biotechnology; Recombinant proteins; CHO Cells; Epithelial Cells; Recombinant Proteins -- biosynthesis; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493969722; 1493969722; 1493969714; 9781493969715
• Related ISBNs: 9781493969715
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed May 18, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.