Metagenomics : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Wolfgang R. Streit, Microbiologie & Biotechnologie, Biocenter Klein Flottbek, University of Hamburg, Hamburg, Germany, Rolf Daniel, Göttingen Genomics Laboratory, Department of Genomic and Applied Microbiology, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.
• Editors: Streit, Wolfgang R; Daniel, Rolf
• Contents: Preface... Table of Contents... Contributing Authors...1.Construction of Small-Insert and Large-Insert Metagenomic LibrariesCarola Simon and Rolf Daniel2.Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene StudiesDominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer3.Construction and Screening Marine Metagenomic Large Insert LibrariesNancy Weiland-Bräuer, Daniela Langfeldt, and Ruth A. Schmitz4.Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme EnvironmentMikkel A. Glaring, Jan K. Vester, and Peter Stougaard5.DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active MicroorganismsEleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont6.Assessing Bacterial and Fungal Diversity in the Plant's EndosphereBernd Wemheuer and Franziska Wemheuer7.Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial CommunitiesJames Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin8.Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized ProductionYuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anné9.Degradation Network Reconstruction Guided by Metagenomic DataRafael Bargiela and Manuel Ferrer10.Novel Tools for the Functional Expression of Metagenomics DNANadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger11.A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme LibrariesDominique Böttcher, Patrick Zägel, Marlen Schmidt, and Uwe T. Bornscheuer12.Screening for Cellulase Encoding Clones in Metagenomics LibrariesNele Ilmberger and Wolfgang R. Streit13.Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic EnzymesMariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade14.Screening Glycosyltransferases for Polyphenol ModificationsNele Ilmberger and Ulrich Rabausch15.Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles16.Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase ActivityGenis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel17.Activity-Based Screening of Metagenomics Libraries for Hydrogenase EnzymesNicole Adam and Mirjam Perner18.Screening for N-AHSL-Based-Signaling Interfering EnzymesStéphane Uroz and Phil M. Oger19.Mining Microbial Signals for Enhanced Biodiscovery of Secondary MetabolitesF. Jerry Reen, Jose A. Gutiérrez-Barranquero, and Fergal O'Gara.
• Edition: Second edition
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Copyright Date: 2017
• Extent: 1 online resource (303 pages)
• Subjects: 579.135; Microbial genomics
• Languages: English
• ISBNs: 9781493966912
• Related ISBNs: 9781493966899
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.