The mitotic exit network : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Fernando Monje-Casas, Ethel Queralt.
• Editors: Monje-Casas, Fernando; Queralt, Ethel
• Contents: Regulation of Mitotic Exit in Saccharomyces cerevisiae -- Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression -- Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining -- Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators -- Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release -- In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP -- Analysis of Protein-Protein Interactions Between MEN Components -- A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity -- Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain -- A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets -- Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast -- Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy -- Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP) -- Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies -- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays -- Analysis of the Functionality of the Mitotic Checkpoints -- Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions -- Studying the Role of the Mitotic Exit Network in Cytokinesis -- Hippo Signaling in Mitosis: An Updated View in Light of the MEN Pathway.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (x, 281 pages), illustrations (some color)
• Subjects: 571.8/4377; Life sciences; Cell cycle -- Laboratory manuals; Cell Cycle; Cell cycle; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals
• Languages: English
• ISBNs: 9781493965021; 1493965026
• Related ISBNs: 9781493965007; 149396500X
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed November 21, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.