Drug target miRNA : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Marco F. Schmidt, BEROCEUTICA GmbH, Potsdam, Germany.
• Editors: Schmidt, Marco F
• Contents: PART I Reviews 1. MiRNA Targeting Drugs: The Next Blockbusters?Marco F. Schmidt PART II Protocols 2.Functional Analysis of miRNA using DIANA Tools Online SuiteIonnis S. Vlachos and Artemis G. Hatzigeorgiou3. Non-Nucleotide Modification of Anti-miRNA OligonucleotidesKim A. Lennox, Christopher A. Vakulskas, and Mark A. Behlke4. Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In VitroDimitrios G. Zisoulis5. Determination of Anti-miR Association with miRNA/Argonaute Complexes In VivoDimitrios G. Zisoulis6. Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miRJohn R. Androsavich7. Assessing Anti-miR Pharmacology with miRNA Polysome Shift AssayJohn R. Androsavich8. Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer CellsJane Zhao and Andreas G. Bader9. Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like ReceptorsGeneviève Pépin, Jonathan Ferrand, and Michael P. Gantier10. Design of Multimodal Small Molecules Targeting miRNA Biogenesis: Synthesis and In Vitro EvaluationDuc D. Vo and Maria Duca11. Machine Learning Approaches towards Building Predictive Models for Small Molecule Modulators of miRNA and its Utility in Virtual Screening of Molecular DatabasesVinita Periwal and Vinod Scaria12. Identification of Small Molecule Modulators of MicroRNA by Library ScreeningYangchao Chen13. Rapid Generation of miRNA Inhibitor Leads by Bio-Informatics and Efficient High-Throughput Screening MethodsChristopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, and Donald G. Phinney14. Peptide-Based Inhibition of miRNA-Guided Gene SilencingJohannes Danner, Balagopal Pai, Ludwig Wankerl, and Gunter Meister15. Small Molecules Targeting the miRNA Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA ImmunoprecipitationTeresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, and Francesco Fazi16. Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein BindersElena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellisimo, Francesco Fazi, and Gianni Colotti17. Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOsMarco F. Schmidt, Oliver Korb, and Chris Abell18. Elucidating Mechanisms of Molecular Recognition between Human Argonaute and miRNA using Computational ApproachesHanlun Jiang, Lizhe Zhu, Amélie Héliou, Xin Gao, Julie Bernbauer, and Xuhui Huang19. Kinetic Analysis of Target RNA Binding and Slicing by Argonaute 2 ProteinSarah Willkomm and Tobias Restle20. Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-AssaysSarah Willkomm, Adrian Zander, and Diana Grohmann21. Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute ComplexMyung Hyun Jo and Sungchul Hohng.
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Copyright Date: 2017
• Extent: 1 online resource (319 pages)
• Subjects: 572.88; Medicine; MicroRNA -- Laboratory manuals; Drug targeting -- Laboratory manuals; Toxicology; Medical -- Pharmacology; Pharmacology
• Languages: English
• ISBNs: 9781493965632
• Related ISBNs: 9781493965618
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.