Wnt signaling : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Quinn Barrett, Lawrence Lum.
• Editors: Barrett, Quinn; Lum, Lawrence
• Contents: Visualizing Wnt Palmiteoylation in Single Cells -- Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction -- Biochemical Methods to Analyze Wnt Protein Secretion -- Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum -- Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway -- A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells -- Wnt-Dependent Control of Cell Polarity in Cultured Cells -- The Use of Chick Embryos to Study Wnt Signaling/Gradients -- Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors -- Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo -- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts -- Delivery of the Porcupine Inhibitor WNT974 in Mice -- Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis -- Monitoring Wnt/b-Catenin Signaling in Skin -- The Generation of Organoids for Studying Wnt Signaling -- Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells -- Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xi, 198 pages), illustrations (some color) and color still photographic images
• Subjects: 572/.64; Life sciences; Wnt proteins -- Laboratory manuals; Wnt proteins; Wnt Signaling Pathway; Wnt Proteins -- metabolism; Signal Transduction -- physiology; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493963935; 1493963937
• Related ISBNs: 9781493963911; 1493963910
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed September 9, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.