Clostridium difficile : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Adam P. Roberts and Peter Mullany.
• Editors: Roberts, Adam P; Mullany, Peter, 1959-
• Contents: Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates -- Direct PCR-ribotyping of Clostridium difficile -- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data -- Clostridium difficile Genome Editing Using pyrE Alleles -- Use of mCherryOpt Fluorescent Protein in Clostridium difficile -- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile -- Clostridium difficile Adhesins -- Intestinal Epithelial Cell Response to Clostridium difficile Flagella -- Isolating and Purifying Clostridium difficile Spores -- Inducing and Quantifying Clostridium difficile Spore Formation -- Characterization of Functional Prophages in Clostridium difficile -- Induction and Purification of C. difficile Phage Tail-like Particles -- Phage Transduction -- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics -- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection -- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community -- A Practical Method for Preparation of Fecal Microbiota Transplantation -- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine -- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins.
• Edition: Second edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xi, 292 pages), illustrations (some color)
• Subjects: 579.3/64; Medicine; Clostridium difficile -- Laboratory manuals; Clostridium difficile; Clostridium difficile; Bacteriological Techniques; Immunology; Medical -- Immunology; Immunology; Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493963614; 1493963619
• Related ISBNs: 9781493963591; 1493963597
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed August 19, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.