Chemotaxis : methods and protocols /: methods and protocols. (©2016)

• Record Type: Book
• Further Information: Note: Edited by Tian Jin and Dale Hereld.
• Other Names: Ph.D, Jin, Tian; Hereld, Dale
• Contents: In Situ Assays of Chemotropism during Yeast Mating -- Imaging Polarization in Budding Yeast Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid -- Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells -- Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET -- Wave Patterns in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals -- Chemotactic Blebbing in Dictyostelium Cells -- Dissecting Spatial and Temporal Sensing in Dictyostelium Chemotaxis Using a Wave Gradient Generator -- Employing Dictyostelium as an Advantageous 3Rs Model for Pharmacogenetic Research -- Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis -- Biochemical Responses to Chemically Distinct Chemoattractants during the Growth and Development of Dictyostelium -- Live Imaging of Border Cell Migration in Drosophila -- ShRNA-Induced Gene Knock-Down In Vivo to Investigate Neutrophil Function -- Studying Neutrophil Migration In Vivo Using Adoptive Cell Transfer -- Intravital Two-Photon Imaging of Lymphocytes Crossing High Endothelial Venules and Cortical Lymphatics in the Inguinal Lymph Node -- Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response -- Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D -- 4D Tumorigenesis Model for Quantitating Coalescence, Directed Cell Motility and Chemotaxis, Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs -- An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells and Cell Sheets -- Axon Guidance Studies Using a Microfluidics-Based Chemotropic Gradient Generator -- Visualization of Actin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy -- Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent Microscopy -- Fluorescence Readout of a Patch Clamped Membrane by Laser Scanning Microscopy -- Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization -- Multi-State Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule Imaging Analysis -- Mathematics of Experimentally Generated Chemoattractant Gradients -- Modeling Excitable Dynamics of Chemotactic Networks.
• Edition: 2nd ed
• Publisher Details: New York, NY : Humana Press Springer
• Publication Date: 2016
• Copyright Date: 2016
• Extent: 1 online resource (xiv, 425 pages), illustrations (some color)
• Subjects: 571.9/6; Life sciences; Chemotaxis -- Laboratory manuals; Chemotaxis; Chemotaxis; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493934805; 1493934805; 1493934783; 9781493934782
• Related ISBNs: 9781493934782
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title form PDF title page (SpringerLink, viewed June 15, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.