Nuclear reprogramming : methods and protocols /: methods and protocols. ([2015])

• Record Type: Book
• Further Information: Note: Edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
• Editors: Beaujean, Nathalie; Jammes, Hélène; Jouneau, Alice
• Contents: Nuclear Transfer in the Mouse -- Nuclear Transfer in the Rabbit -- Nuclear Transfer in Ruminants -- Nuclear Transfer and Transgenesis in the Pig -- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation -- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte -- Assessing the Quality of Donor Cells: Karyotyping Methods -- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer -- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos -- Visualization of Epigenetic Modifications in Preimplantation Embryos -- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer -- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming -- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining -- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR) -- Studying Bovine Early Embryo Transcriptome by Microarray -- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level -- Micro Chromatin Immunoprecipitation ({uF06D}ChIP) from Early Mammalian Embryos -- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation -- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology -- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).
• Edition: Second edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2015
• Copyright Date: 2015
• Extent: 1 online resource (xi, 285 pages), illustrations (some color)
• Subjects: 660.6/5; Life sciences; Cell nuclei -- Transplantation -- Laboratory manuals; Cell nuclei -- Transplantation; Cellular Reprogramming; Cell Nucleus -- physiology; Nuclear Transfer Techniques; Science -- Life Sciences -- Genetics & Genomics; Genetic engineering; Cytology; Genetic engineering; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Laboratory manuals; Electronic books; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493915941; 1493915940; 1493915932; 9781493915934
• Related ISBNs: 9781493915934
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed October 13, 2014).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.