Pancreatic islets isolation : from the mouse to the clinic /: from the mouse to the clinic. (2016)

• Record Type: Book
• Further Information: Note: Miriam Ramírez-Domínguez, editor.
• Editors: Ramírez-Domínguez, Miriam
• Contents: Dedication; Preface; Contents; Contributors; 1: Historical Background of€Pancreatic Islet Isolation; 1.1 The History of€Diabetes Mellitus; 1.1.1 Discovery of€Insulin; 1.2 The History of€Islet Isolation; 1.3 Concluding Remarks; References; 2: The Different Faces of€the€Pancreatic Islet; 2.1 Introduction; 2.1.1 Cytoarchitecture; 2.1.2 Vasculature; 2.1.3 Paracrine Signaling; 2.1.4 Innervation; 2.2 Summary; References; 3: Isolation of€Mouse Pancreatic Islets of€Langerhans; 3.1 Introduction; 3.2 Pancreas Distention and€Dissection; 3.2.1 General Considerations About€the€Procedure. 3.2.2 Enzyme Administration3.3 Pancreas Digestion; 3.3.1 General Considerations About€the€Procedure; 3.3.2 Selection of€the€Enzyme; 3.3.3 Duration of€Digestion; 3.4 Islet Purification; 3.4.1 General Considerations About€the€Procedure; 3.4.2 Density Gradient Purification; 3.4.3 Other Purification Methods; 3.5 Islet Culture; 3.5.1 General Considerations About€the€Procedure; 3.6 Concluding Remarks; References; 4: Pancreatic Islets: Methods for€Isolation and€Purification of€Juvenile and€Adult Pig Islets; 4.1 Introduction; 4.2 Pig Donor Variables; 4.2.1 Pig Strain. 4.2.2 Porcine Donor Age4.3 Pig Pancreas Procurement; 4.3.1 Intraductal Pancreas Perfusion Technique; 4.3.2 Intraductal Pancreas Perfusion Buffer; 4.4 Pig Pancreas Digestion; 4.4.1 Enzyme Blend Selection; 4.4.2 Dissociation; 4.5 Porcine Islet Purification; 4.5.1 Determinants of€Intrinsic Pig Pancreas Density; 4.5.2 Reversing Cell Swelling; 4.5.3 Selection of€Density Gradient Media; 4.5.4 Density Gradient Centrifugation Technique; 4.5.5 Isokinetic Islet Purification; 4.6 Porcine Islet Culture; 4.6.1 Selecting the€Most Suitable Culture Medium; 4.6.2 Glucose Concentration. 4.6.3 Serum Content4.6.4 Glutamine Supplementation; 4.6.5 Culture Temperature; 4.7 Summary; References; 5: Isolation of€Pancreatic Islets from€Nonhuman Primates; 5.1 Introduction; 5.2 Description of€the€Procedure and€Key Aspects; 5.2.1 Laboratory Set Up; 5.2.1.1 Procedures Prior to€the€Day of€Islet Isolation; 5.2.1.2 Procedures on€the€Day of€Islet Isolation; Solutions and€Instruments; Preparation of€Digestive Enzymes; Pancreas Procurement; Collagenase Digestion and€Dilution; Concentration and€Purification of€Islets; Determination of€Islet Yield and€Purity. Islet Assessment in€NHP IsletsIn Vitro Tests; In Vivo Test; 5.3 Conclusions; References; 6: Necessities for€a€Clinical Islet Program; 6.1 Introduction; 6.2 The Islet Processing Facility; 6.3 Clinical Islet Isolation Outcomes; 6.4 The Donor Organ; 6.4.1 Effect of€Donor Age; 6.4.2 Effect of€BMI; 6.4.3 Effect of€Cold Ischaemic Time (CIT); 6.5 Organ Retrieval; 6.6 Pancreas Preservation; 6.6.1 The Two-Layer Method; 6.6.2 Machine Perfusion; 6.7 The Pancreas; 6.8 Concluding Remarks; References; 7: Clinical Islet Isolation.
• Publisher Details: Switzerland : Springer
• Publication Date: 2016
• Extent: 1 online resource
• Subjects: 612.34; Life sciences; Islands of Langerhans; Cell separation; MEDICAL -- Physiology; SCIENCE -- Life Sciences -- Human Anatomy & Physiology; Cell separation; Islands of Langerhans; Islands of Langerhans; Technology & Engineering -- Engineering (General); Medical -- Endocrinology & Metabolism; Science -- Life Sciences -- Zoology -- General; Biomedical engineering; Endocrinology; Animal pathology & diseases; Cytology; Biomedical engineering; Endocrinology; Laboratory animals; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books
• Languages: English
• ISBNs: 9783319398242; 3319398245
• Related ISBNs: 9783319398228; 3319398229
• Notes: Note: Includes bibliographical references and index.; Note: Online resource, title from PDF title page (EBSCO), viewed September 12, 2016.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.