Genome editing. (2016)

• Record Type: Book
• Further Information: Note: Kursad Turksen, editor.
• Editors: Turksen, Kursad
• Contents: Contributors; Genome Editing with Targetable Nucleases; Introduction; Zinc-Finger Nucleases; Transcription Activator-Like Effector Endonucleases; RNA-Guided Nucleases; Target Specificity; Genome Editing with Targetable Nucleases; Promoting Homologous Recombination over Nonhomologous End-Joining; Delivery Methods; Applications; Conclusion; References; CRISPR/Cas9 Approaches to Investigate the Noncoding Genome; Introduction; Genetic Manipulations of Noncoding Sequences in the Prenuclease Era; Editing by Chromosomal Deletion; Epigenome Editing; Targeting Noncoding RNAs ConclusionReferences; At the Conflux of Human Genome Engineering and Induced Pluripotency; Induced Pluripotency and the Human Genetic Model Organism In Vitro; Rise of the Genome Editing Machines; Adding Function to iPSCs Through Transgenesis at Safe-­Harbor Loci; Achieving Seamless Genome Engineering for Accurate Disease Models; Avoiding Unwanted Outcomes: Off-Target Cleavage and Mosaicism; Selection of Isogenic Clones and Technical Controls; Conclusions; References; CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies; Introduction Traditional Mouse Genome Engineering Technologies in the Pre-CRISPR Era Traditional Tg Techniques and Their Limitations; Traditional KO/KI Techniques and Their Limitations; Microinjection and Its Limitations; CRISPR/Cas9 and Mouse Genome Editing; CRISPR Technology and the Paradigm Shifts in Mouse Genome Engineering; The Current Challenges of CRISPR/Cas9-Mediated Mouse Genome Engineering; Poor Efficiency of Insertion of Sequences at Cas9 Cut Sites; Challenges in Developing Conditional KO Models; Off-Target Effects; Challenges Associated with Genotyping Future Impact of CRISPR/Cas9 on Manipulating the Mouse Genome Impact on Random Tg Technologies; Impact on KO/KI Technologies; Impact on Microinjection Technique; Conclusion; References; Genome-Editing Technology in CRISPR/Cas System: How to Increase Knock-In Efficiency in Mouse Zygotes; Introduction; CRISPR/Cas System; Delivery System of Cas9 and gRNA; Single-Stranded Oligo-DNA and Double-Stranded DNA as Donor Templates; Molecules That Increase HDR Efficiency; Cell Cycle; Perspective; Conclusion; References; Developments in the Generation of Reporter Stem Cells; Introduction Gene Reporter Pluripotent Lines as Cell Models Methods for the Generation of Reporter Pluripotent Stem Cells; Minimal Promoter-Driven Reporter Expression; Nonintegrational Methods; Integrational Methods; Reporter Knock-In into Endogenous Promoter Site; Homologous Recombination; Mega Nucleases; Zinc-Finger Nucleases; TALEN; CRISPR; Alternate Approaches; Potential Uses of Reporter Stem Cells; References; Current Status of Genome Editing in Cardiovascular Medicine; Cardiovascular Disease Genomics; Precision Medicine; The Genetics of Cardiovascular Disease
• Publisher Details: Switzerland : Springer
• Publication Date: 2016
• Extent: 1 online resource (ix, 166 pages), color illustrations
• Subjects: 576.5; 570; Life sciences; Genetic engineering; Stem cells; SCIENCE / Life Sciences / Evolution; Genetic engineering; Stem cells; Genetic Engineering; Science -- Life Sciences -- Cytology; Science -- Life Sciences -- Zoology -- General; Genetics (non-medical); Animal reproduction; Animal genetics; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Electronic books
• Languages: English
• ISBNs: 9783319341484; 3319341480
• Related ISBNs: 9783319341460; 3319341464
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed August 24, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.