Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases. (2016)

• Record Type: Book
• Further Information: Note: Matthew Jenner.
• Authors: Matthew, Jenner
• Contents: Parts of this thesis have been published in the following journal articles:; Supervisor's Foreword; Abstract; Acknowledgements; Contents; Abbreviations; 1 Introduction; 1.1 Natural Products; 1.1.1 Polyketides and Non-ribosomal Peptides; 1.2 Polyketide Synthases; 1.2.1 Polyketide Biosynthesis; 1.2.2 Polyketide Synthase Classification; 1.2.2.1 Type I PKSs; Iterative Type I PKSs; Modular Type I PKSs; The Principle of Colinearity; cis- and trans-AT PKSs; KS Specificity-Based Assignment of trans-AT PKSs; 1.2.2.2 Type II PKSs; 1.2.2.3 Type III PKSs; 1.2.2.4 Non-ribosomal Peptide Synthases. 1.2.2.5 Hybrid NRPS-PKS Systems1.2.2.6 Bacillaene: Properties, Structure and Biosynthesis; 1.2.2.7 Psymberin: Properties, Structure and Biosynthesis; 1.2.3 Structure and Mechanisms of PKS Domains; 1.2.3.1 The Ketosynthase Domain; 1.2.3.2 Loading and Non-elongating Ketosynthase Domains; 1.2.3.3 The Acyltransferase Domain; 1.2.3.4 The Acyl Carrier Protein; 1.2.3.5 The Ketoreductase, Dehydratase and Enoylreductase Domains; 1.2.3.6 The Thioesterase Domain; 1.2.4 Architechture of the 'Megasynthase'; 1.2.5 In Vitro Techniques for Studying PKSs; 1.3 Mass Spectrometry; 1.3.1 Ionisation Methods. 1.3.1.1 Electrospray Ionisation1.3.1.2 Nano-Electrospray Ionisation; 1.3.1.3 Analysing Proteins by ESI-MS; 1.3.2 Mass Analysers; 1.3.2.1 Quadrupole Analysers; 1.3.2.2 Time-of-Flight Analysers; 1.3.2.3 Hybrid Mass Analyser Instruments; 1.3.2.4 Tandem Mass Spectrometry; Collision Induced Dissociation; References; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments; 2.1.1.1 Protein Production, Purification and Mutagenesis; 2.1.1.2 Mass Spectrometry; Waters SYNAPT HDMS; Nanospray Capillaries; 2.1.2 Buffers and Reagents; 2.1.2.1 Reagents; 2.1.2.2 Transformation and Expression Media. Cloning of PsyA ACP1-KS1 Didomain2.2.2 Synthesis of N-Acetylcysteamine Thioesters; 2.2.3 Ketosynthase Acylation Assay; 2.2.4 Synthesis of Acyl-Acyl Carrier Proteins; 2.2.5 Acyl-ACP Ketosynthase Loading Assay; 2.2.6 PedCPedD(R97Q) Hydrolase Assays; 2.2.7 Acyltransferase Extender Unit Specificity Assays; 2.2.8 PedD Malonyl Loading and Unloading of ACP; 2.2.9 Ketosynthase Elongation Assay; 2.2.9.1 Synthesis of Malonyl-ACP; 2.2.9.2 Synthesis of Alkyl-ACP; 2.2.9.3 Ketosynthase Elongation Reactions and MS Analysis; 2.2.10 Monitoring Acyl-Transfer in PsyA ACP1-KS1.
• Publisher Details: Switzerland : Springer
• Publication Date: 2016
• Extent: 1 online resource (xviii, 176 pages), illustrations (some color)
• Subjects: 572/.45; Chemistry; Polyketides -- Spectra; Mass spectrometry; SCIENCE -- Life Sciences -- Biochemistry; Mass spectrometry; Technology & Engineering -- Chemical & Biochemical; Medical -- Biochemistry; Enzymology; Biochemical engineering; Biochemistry; Enzymes; Biochemical engineering; Biochemistry; Science -- Spectroscopy & Spectrum Analysis; Spectrum analysis, spectrochemistry, mass spectrometry; Electronic books
• Languages: English
• ISBNs: 9783319327235; 3319327232; 3319327224; 9783319327228
• Related ISBNs: 9783319327228
• Notes: Note: Includes bibliographical references.; Note: Online resource; title from PDF title page (SpringerLink, viewed May 5, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.