Bacterial multidrug exporters : methods and protocols /: methods and protocols. (2018)

• Record Type: Book
• Further Information: Note: Edited by Akihito Yamaguchi, Kunihiko Nishino.
• Editors: Yamaguchi, Akihito; Nishino, Kunihiko
• Contents: High Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins) -- Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically-Relevant Asymmetric Crystals -- Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors -- Crystallographic Analysis of the CusBA Heavy-Metal Efflux Complex of Escherichia coli.- Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis -- NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE -- Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA -- Biochemical Reconstitution and Characterization of Multi-Component Drug Efflux Transporters -- Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Their Mechanism of Action: Escherichia coli AcrB Multidrug Exporter as an Example -- Determining Ligand Path through a Major Drug Transporter, AcrB, in Escherichia coli.- Molecular Modeling of Multi-Drug Properties of Resistance Nodulation Division (RND) Transporters -- A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria -- Regulation of the Expression of Bacterial Multidrug Exporters by Two-Component Signal Transduction Systems -- Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions -- Identification of a Staphylococcus aureusEfflux Pump Regulator Using a DNA-Protein Affinity Technique -- High Throughput Flow Cytometry Screening of Multidrug Efflux Systems -- Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem -- Large Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria -- Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2018
• Copyright Date: 2018
• Extent: 1 online resource (xii, 355 pages), illustrations (some color)
• Subjects: 572/.696; Medicine; Membrane proteins -- Laboratory manuals; Multidrug resistance -- Laboratory manuals; Membrane proteins; Multidrug resistance; Multidrug Resistance-Associated Proteins -- analysis; Drug Resistance, Multiple, Bacterial; Immunology; Medical -- Immunology; Immunology; Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493974542; 1493974548
• Related ISBNs: 9781493974528; 1493974521
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed December 4, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.