Synthetic antibodies : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Thomas Tiller.
• Editors: Tiller, Thomas
• Contents: Part I: Amino Acid-Based Synthetic Antibodies 1. Antibody Mimetics, Peptides, and Peptidomimetics Xiaoying Zhang and Thirumalai Diraviyam 2. Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity Xuelian Bai and Hyunbo Shim 3. Enzymatic Assembly for scFv Library Construction Mieko Kato and Yoshiro Hanyu 4. Directed Evolution of Protein Thermal Stability Using Yeast Surface Display Michael W. Traxlmayr and Eric V. Shusta 5. Whole Cell Panning with Phage Display Yvonne Stark, Sophie Venet, and Annika Schmid 6. Generating Conformation and Complex-Specific Synthetic Antibodies Marcin Paduch and Anthony A. Kossiakoff 7. High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast Andrea Sterner and Carolin Zehetmeier 8. Utilization of Selenocysteine for Site-Specific Antibody Conjugation Xiuling Li and Christoph Rader 9. Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions Emilie Renaud, Pierre Martineau, and Laurence Guglielmi 10. Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis Helena Persson, Charlotta Preger, Edyta Marcon, Johan Lengqvist, and Susanne Gräslund 11. Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules Neeraj Kohli and Melissa L. Geddie 12. Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells Kai Zhang, Stephen J. Demarest, Xiufeng Wu, and Jonathan R. Fitchett 13. A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells Hongkai Zhang and Richard A. Lerner 14. A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins Wouter P.R. Verdurmen, Marigona Mazlami, and Andreas Plückthun 15. Data-Driven Antibody Engineering Using Genedata Biologics™ Maria Wendt and Guido Cappuccilli Part II: Nucleotide-Based Synthetic Antibodies: Aptamers 16. Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers Nam Nguyen Quang, Anna Miodek, Agnes Cibiel, and Frédéric Ducongé 17. Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules Grigory S. Filonov 18. An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding Matthew D. Moore, Blanca I. Escudero, and Lee-Ann Jaykus 19. Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein Elyse D. Bernard, Kathy C. Nguyen, Maria C. DeRosa, Azam F. Tayabali, and Rocio Aranda-Rodriguez Part III: Moleculary Imprinted Polymers 20. Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer Romana Schirhagl 21. Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization Tibor Renkecz and Viola Horvath 22. Generation of Janus Molecularly Imprinted Polymer Particles Xiantao Shen and Chuixiu Huang 23. Surface Engineering of Nanoparticles to Create Synthetic Antibodies Linda Chio, Darwin Yang, and Markita Landry 24. H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers Chak Sangma, Peter A. Lieberzeit, and Wannisa Sukjee 25. Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection Francesco Canfarotta, Katarzyna Smolinska-Kempisty, and Sergey Piletsky 26. Cell and Tissue Imaging with Molecularly Imprinted Polymers Maria Panagiotopoulou, Stephanie Kunath, Karsten Haupt, and Bernadette Tse Sum Bui.
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2017
• Extent: 1 online resource (xiv, 420 pages), illustrations (some color)
• Subjects: 571.9/67; Medicine; Immunoglobulins -- Laboratory manuals; Immunoglobulins -- Biotechnology -- Laboratory manuals; Synthetic biology -- Laboratory manuals; Immunoglobulins; Immunoglobulins -- Biotechnology; Synthetic biology; Antibodies; Antibodies, Monoclonal; Synthetic Biology; Immunology; Medical -- Immunology; Immunology; Electronic books; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493968572; 1493968572
• Related ISBNs: 9781493968558; 1493968556
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title form PDF title page (SpringerProtocols, viewed March 6, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.