Signal transduction immunohistochemistry : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Alexander E. Kalyuzhny.
• Editors: Kalyuzhny, Alexander E
• Contents: Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications -- Antibodies That Work Again and Again and Again -- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods -- Acquisition and Post-Processing of Immunohistochemical Images -- Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction -- EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis -- Preservation of Specific Protein Signaling States using Heat Based Stabilizor System -- Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species -- Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages -- Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers -- A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy -- Apoptotic Bodies: Selective Detection in Extracellular Vesicles -- Activation and Differentiation of Mesenchymal Stem Cells -- High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP -- Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry -- Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D -- Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment -- Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors -- Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt -- Automated High Content Screening of g -- H2AX Expression in HeLa Cells.
• Edition: Second edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Extent: 1 online resource (x, 290 pages), illustrations (some color)
• Subjects: 571.7/4; Medicine; Cellular signal transduction; Immunohistochemistry; Cellular signal transduction; Immunohistochemistry; Mechanotransduction, Cellular; Immunohistochemistry -- methods; Immunology; Medical -- Immunology; Immunology; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781493967599; 1493967592
• Related ISBNs: 9781493967575; 1493967576
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed February 15, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.