Bacterial protein secretion systems : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Laure Journet, Eric Cascales.
• Editors: Journet, Laure; Cascales, Eric
• Contents: 1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder Sophie S Abby and Eduardo PC Rocha 2. Protein Sorting Prediction Henrik Nielsen 3. Cell Fractionation Melissa Petiti, Laetitia Houot, and Denis Duché 4. Defining Lipoprotein Localisation by Fluorescence Microscopy Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée 5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate Nienke Buddelmeijer 6. Defining Membrane Protein Localization by Isopycnic Density Gradients Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow 7. Components Sub-cellular Localisation: Cell Surface Exposure Anna Konovalova 8. Probing Inner Membrane Protein Topology by Proteolysis Maxence S. Vincent and Eric Cascales 9. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM) Mikhail Bogdanov 10. Defining Membrane Protein Topology using pho-lac Reporter Fusions Gouzel Karimova and Daniel Ladant 11. In vivo and in vitro Protein-peptidoglycan Interactions Gang Li and S. Peter Howard 12. Measure of Peptidoglycan Hydrolase Activity Yoann G. Santin and Eric Cascales 13. Protein-protein Interaction: Bacterial Two-Hybrid Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant 14. Protein-protein Interactions: Yeast two-hybrid Jer-Sheng Lin and Erh-Min Lai 15. Protein-protein Interactions: Cytology Two-hybrid Krishnamohan Atmakuri 16. Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes Laureen Logger, Abdelrahim Zoued, and Eric Cascales 17. Protein-protein Interactions: Co-immunoprecipitation Jer-Sheng Lin and Erh-Min Lai 18. Protein-protein Interaction: Tandem Affinity Purification in Bacteria Julie P.M. Viala and Emmanuelle Bouveret 19. Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes Raffaele Ieva 20. Protein-protein Interactions: Pull-down Assays Arthur Louche, Suzana P. Salcedo, and Sarah Bigot 21. Protein-protein interactions: Surface Plasmon Resonance Badreddine Douzi 22. Assessing Energy-dependent Protein Conformational Changes in the TonB System Ray A. Larsen 23. Defining Assembly Pathways by Fluorescence Microscopy Abdelrahim Zoued and Andreas Diepold 24. Large Complexes: Cloning Strategy, Production, and Purification Eric Durand and Roland Lloubes 25. Shearing and Enrichment of Extracellular Type IV Pili Alba Katiria, Gonzalez Rivera, and Katrina T. Forest 26. Blue Native PAGE Analysis of Bacterial Secretion Complexes Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner 27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer 28. Structural Analysis of Protein Complexes by Cryo Electron Microscopy Tiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova 29. Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy Birgit Habenstein and Antoine Loquet 30. Identificationof Effectors: Precipitation of Supernatant Material Nicolas Flaugnatti and Laure Journet 31. Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System Marc Erhardt 32. Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot Brent S. Weber, Pek Man Ly, and Mario F. Feldman 33. Effector Translocation: CyaReporter Assay Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko 34. Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System Julie Allombert, Anne Vianney, and Xavier Charpentier 35. Effector Translocation Assay: Differential Solubilization Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota 36. Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture Juliana Alcoforado Diniz, Birte Hollmann, and Sarah Coulthurst.
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (528 pages)
• Subjects: 572.64; Life sciences; Protein-protein interactions -- Research -- Methodology; Secretion; Microbiology; Science -- Life Sciences -- Biology -- Microbiology; Microbiology (non-medical)
• Languages: English
• ISBNs: 9781493970339
• Related ISBNs: 9781493970315
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.