Tau protein : methods and protocols /: methods and protocols. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Caroline Smet-Nocca.
• Editors: Smet-Nocca, Caroline
• Contents: Conformational Dynamics of Intracellular Tau Protein Revealed by CD and SAXS -- Global Conformation of Tau Protein Mapped by Raman Spectroscopy -- Molecular Dynamics Simulation of Tau Peptides for the Investigation of Conformational Changes Induced by Specific Phosphorylation Patterns -- Tau Interaction with Tubulin and Microtubules: From Purified Proteins to Cells -- X-Ray Structural Study of Amyloid-Like Fibrils of Tau Peptides Bound to Small-Molecule Ligands -- Detection and Quantification Methods for Fibrillar Products of In Vitro Tau Aggregation Assays -- Fourier Transform Infrared (FTIR) Spectroscopy, Ultraviolet Resonance Raman (UVRR) Spectroscopy and Atomic Force Microscopy (AFM) for Study of the Kinetics of Formation and Structural Characterization of Tau Fibrils -- Assays for the Screening and Characterization of Tau Aggregation Inhibitors -- Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo -- Mass Spectrometry Analysis of Lysine Post-Translational Modifications of Tau Protein from Alzheimer’s Disease Brain -- The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein -- Tag-Free Semi-Synthesis of Tau Protein -- Production of O-GlcNAc Modified Recombinant Tau in E.coli and Detection of Ser400 O-GlcNAc Tau In Vivo -- Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources -- A Simple Method to Avoid Non-Specific Signal When Using Monoclonal Anti-Tau Antibodies in Western Blotting of Mouse Brain Proteins -- Flow Cytometry Analysis and Quantitative Characterization of Tau in Synaptosomes from Alzheimer’s Disease Brains -- In Vivo Microdialysis of Brain Interstitial Fluid for the Determination of Extracellular Tau Levels -- Proximity Ligation Assay: A Tool to Study Endogenous Interactions between Tau and its Neuronal Partners -- Finding MAPT Mutations in Frontotemporal Dementia and Other Tauopathies -- Tracking Tau in Neurons: How to Grow, Fix and Stain Primary Neurons for the Investigation of Tau in All Developmental Stages -- Tracking Tau in Neurons: How to Transfect and Track Exogenous Tau into Primary Neurons -- Image-Based Analysis of Intracellular Tau Aggregation by Using Tau-BiFC Cell Model -- FRET and Flow Cytometry Assays to Measure Proteopathic Seeding Activity in Biological Samples -- In Vivo Imaging of Tau Aggregates in the Mouse Retina -- In Vivo Hyperthermic Stress Model: An Easy Tool to Study the Effects of Oxidative Stress on Neuronal Tau Functionality in Mouse Brain -- Identification of Tau Toxicity Modifiers in the Drosophila Eye -- Regulation of Neurotrophic Factors during Pathogenic Tau-Aggregation: A Detailed Protocol for Double-Labeling mRNA by In Situ Hybridization and Protein Epitopes by Immunohistochemistry -- Pin1 Knockout Mice: A Model for the Study of Tau Pathology in Alzheimer's Disease.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (xviii, 432 pages), illustrations (some color)
• Subjects: 572/.6; Life sciences; Proteins -- Laboratory manuals; Proteins; tau Proteins; Alzheimer Disease -- pathology; Microtubules -- metabolism; Tubulin -- metabolism; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493965984; 1493965980
• Related ISBNs: 9781493965960; 1493965964
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed December 21, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.