Programmed cell death : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Hamsa Puthalakath and Christine J. Hawkins.
• Editors: Puthalakath, Hamsa; Hawkins, Christine J
• Contents: Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry -- In Vivo Apoptosis Imaging Using Site-Specifically 68Ga-Labeled Annexin V -- Detection of Active Caspases during Apoptosis Using Fluorescent Activity-Based Probes -- Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation -- In Vitro Use of Peptide Based Substrates and Inhibitors of Apoptotic Caspases -- Experimental In Vivo Sepsis Models to Monitor Immune Cell Apoptosis and Survival in Laboratory Mice -- Analysis of Cell Death Induction in Intestinal Organoids In Vitro -- In Vitro Differentiation of Mouse Granulocytes -- Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection -- Isolation of Cardiomyocytes and Cardiofibroblasts for Ex-Vivo Analysis -- Detection of Cell Death in Drosophila Tissues -- Methods to Study Plant Programmed Cell Death -- Modeling Metazoan Apoptotic Pathways in Yeast -- Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE -- Using Förster-Resonance Energy Transfer to Measure Protein Interactions between Bcl-2 Family Proteins on Mitochondrial Membranes -- Preparing Samples for Crystallization of Bcl-2 Family Complexes -- Screening Strategies for TALEN Mediated Gene Disruption -- Using CRISPR/Cas9 Technology for Manipulating Cell Death Regulators -- Lentiviral Vectors to Analyze Cell Death Regulators -- Proteomic Profiling of Cell Death: Stable Isotope Labeling and Mass Spectrometry Analysis. .
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xi, 291 pages), illustrations (some color)
• Subjects: 571.9/36; Life sciences; Cell death -- Laboratory manuals; Cell death; Cell Death; Apoptosis; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Laboratory manuals; Electronic books; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493935819
• Related ISBNs: 149393581X; 9781493935796; 1493935798
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed April 25, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.