High-resolution imaging of cellular proteins : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.
• Editors: Schwartzbach, Steven D; Skalli, Omar; Schikorski, Thomas
• Contents: Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture -- Antibody Production with Synthetic Peptides -- Production and Purification of Polyclonal Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin -- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications -- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy -- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells -- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses -- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells -- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging -- Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins -- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas -- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues -- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae -- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes -- Pre-Embedding Nanogold Silver and Gold Intensification -- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval -- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections -- Immuno-Gold Labelling for Scanning Electron Microscopy -- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope -- Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xiii, 366 pages), illustrations (some color)
• Subjects: 502.8/25; Medicine; Electron microscopy -- Laboratory manuals; Electron microscopy; Proteins -- ultrastructure; Image Enhancement -- methods; Cells -- cytology; Microscopy, Confocal -- methods; Microscopy, Electron -- methods; Structure-Activity Relationship; Radiology, Medical; Medical -- Biochemistry; Radiology; Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493963522
• Related ISBNs: 149396352X; 9781493963508; 1493963503
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed August 19, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.