Natural killer cells : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Srinivas S. Somanchi.
• Editors: Somanchi, Srinivas S
• Contents: Tracking Effector and Memory NK Cells During MCMV Infection -- Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells -- Assessment of NK Cell Metabolism -- Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK Cells -- Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH) -- In Vitro Assessment of Human Natural Killer Cell Migration and Invasion -- Microfluidic-Based Live-Cell Analysis of NK-Cell Migration In Vitro -- Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response -- Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method -- Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry -- Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor Signaling -- High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse -- The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunological Synapses and their Functions -- Expansion of NK Cells Using Genetically Engineered K562 Feeder Cells -- Ex-Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21 -- Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy -- Gene Modification of Human Natural Killer Cells Using a Retroviral Vector -- Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular Therapy -- mRNA Transfection to Improve NK Cell Homing to Tumors -- In Vitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor through Differentiation of Gene-Modified Hematopoietic Stem Cells -- Engineering Receptor Expression on Natural Killer Cells through Trogocytosis -- Electroporation of siRNA to Silence Gene Expression in Primary NK Cells -- Mouse Xenograft Model for Intraperitoneal Administration of NK Cell Immunotherapy for Ovarian Cancer -- Aerosol Delivery of Interleukin-2 in Combination with Adoptive Transfer of Natural Killer Cells for the Treatment of Lung Metastasis: Methodology and Effect -- Non-Invasive Imaging of Natural Killer Cell-Mediated Apoptosis in a Mouse Tumor Model -- Non-Invasive In Vivo Fluorescence Imaging of NK Cells in Pre-Clinical Models of Adoptive Immunotherapy -- In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells -- Generation of BiKEs and TriKEs to Improve NK Cell-Mediated Targeting of Tumor Cells -- Regulatory Considerations for NK Cells Used in Human Immunotherapy Applications.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xv, 365 pages), illustrations (some color)
• Subjects: 571.9/68; Medicine; Killer cells -- Laboratory manuals; Killer cells; Killer Cells, Natural; Immunology; Medical -- Immunology; Immunology; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493936847; 1493936840; 1493936824; 9781493936823
• Related ISBNs: 9781493936823
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed May 17, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.