High-throughput RNAi screening : methods and protocols /: methods and protocols. ([2016])

• Record Type: Book
• Further Information: Note: Editors David O. Azorsa, Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, Arizona, USA; Shilpi Arora, Constellation Pharmaceuticals, Cambridge, Massachusetts, USA.
• Editors: Azorsa, David O; Arora, Shilpi
• Contents: Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization of Transfection Conditions for siRNA Screening -- High Throughput siRNA Screening Using Reverse Transfection -- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells -- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform -- A Protocol for a High-Throughput Multiplex Cell Viability Assay -- RNAi Screening of Leukemia Cells Using Electroporation -- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening -- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity -- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens -- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development -- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans -- Design and Methods of Large-Scale RNA Interference Screens in Drosophila -- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity -- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae -- RNAi Screening in Spodoptera frugiperda -- Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos -- Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens -- Data Analysis For High Throughput RNAi Screening. .
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xi, 262 pages), illustrations (some color)
• Subjects: 572.8/8; Medicine; RNA interference -- Laboratory manuals; High throughput screening (Drug development) -- Laboratory manuals; High throughput screening (Drug development); RNA interference; RNA Interference; Drug Evaluation, Preclinical -- methods; Drug Discovery; Human genetics; Medical -- Genetics; Medical genetics; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493963379; 1493963376
• Related ISBNs: 9781493963355; 149396335X
• Notes: Note: Includes bibliographical references and index.; Note: Description based on online resource; title from PDF title page (SpringerLink, viewed September 22, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.